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- Flow Cytometry Basics
- Flow Cytometry Principles
- Identifying and Using Cell Surface Markers
- Identifying General Cell Types Associated With Surface Markers
- Interpreting Instrument Printouts
- Review of Flow Cytometry Principles
- Principles of Flow Cytometry
- Case Study #1
- Case One
- Scatter and CD45
- What cell population is gated in this peripheral blood sample case analyzed by flow cytometry?
- CD19 and CD20
- It has already been established that the gated cell population is lymphocytic in nature. CD19 and CD20 cell surface antigens both appear on what type ...
- CD10, HLA-DR, Kappa, and Lambda
- If 100% of the cells in the gated lymphocyte population are positive for CD45 (i.e., they are leukocytes) and 7% are B cells (represented by CD19 and ...
- T Cell Markers
- All helper cells are T cells. Consequently, in a normal lymphocyte population, it is reasonable to expect all CD4-positive cells to be CD3-positive as...
- Final Interpretation of Case #1
- Case Study #2
- Case Two
- Scatter and Gating
- T Cell Analysis
- B Cell Analysis and Final Interpretation of Case Two
Level of instruction: Intermediate
Intended Audience: Medical laboratory scientists, medical technologists, and technicians. This course is also appropriate for medical laboratory science students and pathology residents.
Author information: Dana L. Van Laeys, MA, CLSpMB, MT, CLS is the Education Coordinator for Molecular Diagnostics and Immunology in the Clinical Laboratory Science Program at Saint Luke’s Hospital in Kansas City, Missouri. She has 14 years of experience in molecular diagnostics and flow cytometry. She has been a presenter at ASM, CLEC, and AABB conferences. Ms. Van Laeys holds a Masters in Adult Education and Distance Learning.
Reviewer information: Leah Coppolino, MPH, MT(ASCP), CLS(NCA) currently works for MediaLab, Inc. Previously, she was the Director of the Medical Laboratory Science Program and Director of Education and Outreach at St. Christopher's Hospital for Children in Philadelphia, Pennsylvania. She holds a Masters in Public Health from Thomas Jefferson University.
Course description: This course describes the most basic principles of flow cytometry, providing an overview and introduction to this useful tool. The focus of the course is blood cell identification, specifically B and T lymphocyte identification and analysis.