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Introduction to Flow Cytometry: Blood Cell Identification (Online CE Course)

(based on 491 customer ratings)

Author: Dana L. Van Laeys, MA, CLSpMB, MT, CLS
Reviewer: Leah Coppolino, MPH, MT(ASCP), CLS(NCA)

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No other laboratory method provides as rapid and detailed analysis of cellular populations as flow cytometry, making it a valuable tool for diagnosis and management of several hematologic and immunologic diseases. Understanding this relevant methodology is important for any medical laboratory scientist.

Whether you have no previous experience with flow cytometry or just need a refresher, this course will help you to understand the basic principles, with the help of video tutorials and interactive case studies.

Basic principles include:

Immunophenotypic features of various types of hematologic cells

Labeling cellular elements with fluorochromes

Blood cell identification, specifically B and T lymphocyte identification and analysis

Cell sorting to isolate select cell population for further analysis

Analyzing and interpreting result reports and printouts

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)

Course number 578-018-16, approved through 3/31/2018

Florida Board of Clinical Laboratory Personnel Credit Hours - General (Hematology): 2 hour(s)

Course number 20-551382, approved through 9/1/2018

Objectives

List the appropriate specimen types used for flow cytometric analysis.
Describe the steps involved in analyzing samples by flow cytometry.
Calculate absolute cell counts from percentage values for flow cytometry analysis.
Identify blood cell types associated with respective specific surface markers.
Interpret basic flow cytometer instrument analysis printouts.
Progress through case studies related to lymphocyte evaluation using flow cytometry while performing final interpretations.

Customer Ratings

(based on 491 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.

Flow Cytometry Basics

Flow Cytometry Course Introduction
Definitions

Flow Cytometry Principles

Acceptable Samples
Sample Preparation
Sample Analysis
Sample Analysis Animation
Detection of Cellular Characteristics
Detection of Intrinsic Cellular Characteristics: Cell Size and Granularity
Detection of Extrinsic Cellular Characteristics: Surface Antigens
Cell size is indicated by forward scatter and granularity is indicated by side scatter in flow cytometry.
Staining Illustration
Staining: Monoclonal Antibody Binding
T-Helper Cell Example
Suppressor Cell Example
Flow Cytometry and Human Immunodeficiency Virus Clinical Application
Calculating Absolute Cell Counts
Calculating Absolute Cell Counts- continued
Which of the following types of lymphocytes express CD4?

Identifying and Using Cell Surface Markers

Identifying General Cell Types Associated With Surface Markers

Interpreting Instrument Printouts

Cytogram for Cellular Scatter: Intrinsic Characteristics
White Blood Cells and Cytogram for Cellular Scatter
Extrinsic Cell Characteristics
Which of these white blood cell populations would have the MOST side scatter when analyzed using flow cytometry?
Gating: Selecting the Population of Interest
Using Cell Surface Markers as Tools For Diagnosis
Diagnostic Process

Review of Flow Cytometry Principles

Principles of Flow Cytometry

Case Study #1

Case One
Scatter and CD45
What cell population is gated in this peripheral blood sample case analyzed by flow cytometry?
CD19 and CD20
It has already been established that the gated cell population is lymphocytic in nature. CD19 and CD20 cell surface antigens both appear on what type ...
CD10, HLA-DR, Kappa, and Lambda
If 100% of the cells in the gated lymphocyte population are positive for CD45 (i.e., they are leukocytes) and 7% are B cells (represented by CD19 and ...
T Cell Markers
All helper cells are T cells. Consequently, in a normal lymphocyte population, it is reasonable to expect all CD4-positive cells to be CD3-positive as...
Final Interpretation of Case #1

Case Study #2

Case Two
Scatter and Gating
T Cell Analysis
B Cell Analysis and Final Interpretation of Case Two

Conclusion

Conclusion

References

References

Additional Information

Level of instruction: Intermediate

Intended Audience: Medical laboratory scientists, medical technologists, and technicians. This course is also appropriate for medical laboratory science students and pathology residents.

Author information: Dana L. Van Laeys, MA, CLSpMB, MT, CLS is the Education Coordinator for Molecular Diagnostics and Immunology in the Clinical Laboratory Science Program at Saint Luke’s Hospital in Kansas City, Missouri. She has 14 years of experience in molecular diagnostics and flow cytometry. She has been a presenter at ASM, CLEC, and AABB conferences. Ms. Van Laeys holds a Masters in Adult Education and Distance Learning.

Reviewer information: Leah Coppolino, MPH, MT(ASCP), CLS(NCA) currently works for MediaLab, Inc. Previously, she was the Director of the Medical Laboratory Science Program and Director of Education and Outreach at St. Christopher's Hospital for Children in Philadelphia, Pennsylvania. She holds a Masters in Public Health from Thomas Jefferson University.

Course description: This course describes the most basic principles of flow cytometry, providing an overview and introduction to this useful tool. The focus of the course is blood cell identification, specifically B and T lymphocyte identification and analysis.