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The page below is a sample from the LabCE course Introduction to Flow Cytometry: Blood Cell Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Cytogram for Cellular Scatter: Intrinsic Characteristics

As cells exit the flow cell of the flow cytometer and intersect the laser beam, intrinsic cellular properties can be reflected, analyzed, and depicted in a scatter plot. In this cytogram, side scatter is on the x axis and forward scatter is on the y axis.
*Note: laboratories can identify which axis they will use for each type of light scatter and this will affect the appearance of the scatter plot.