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The page below is a sample from the LabCE course Introduction to Flow Cytometry: Blood Cell Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Scatter and CD45

Image 1 represents the cell scatter. The gated population is what determines the fluorescent data represented in the second image.

Image 1. Note: FSC-A and SSC-A are abbreviations for forward and side scatter, respectively.

Image 2 contains the fluorescence data from CD45 staining. Note that virtually all cells in the gated population have CD45 surface antigens, and are all leukocytes (white blood cells).
Image 2