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- Introduction to the Intestinal Parasitic Protozoa
- Overview of Diagnostic Techniques
- Types of Diagnostic Techniques
- Collection and Processing for Performing the Microscopic Exam
- Collection and Processing for Performing the Microscopic Exam cont'd - Fixatives
- Concentration Techniques
- How to Perform the Microscopic Exam
- Entero-Test (Duodenal Capsule)
- Molecular Methods
- Antigen and Antibody Detection
- Suggested Protocols for Parasite Recovery
- A direct wet prep for the examination of stool specimens for ova and parasites is a preferred method for parasite detection and identification.
- All of the following are stains that can be used on permanent smears for protozoa except:
- Life Cycles, Epidemiology, Diseases, and Diagnosis of the Amoebae
- Pathogenic vs. Commensal Amoebae
- Entamoeba histolytica Life Cycle
- Entamoeba histolytica Epidemiology and Disease
- Differentiating E. histolytica Trophozoites from the Commensal Amoeba Trophs
- Differentiating E. histolytica Cysts from the Commensal Amoeba Cysts
- Other Diagnostic Techniques for Entamoeba histolytica
- Match the amoeba species with whether it is pathogenic or commensal.
- People become infected with Entamoeba histolytica by consuming food contaminated (usually of human fecal origin) with trophozoites of the organism.
- A MLS is puzzled when she finds cysts resembling either E. histolytica or E. hartmanni. A way of distinguishing Entamoeba histolytica cysts from cysts...
- Common methods of detecting pathogenic gastrointestinal infection with Entamoeba histolytica include all of the following except:
- Life Cycles, Epidemiology, Diseases, and Diagnosis of the Flagellates
- Pathogenic vs. Commensal Flagellates
- Giardia and Dientamoeba Life Cycles
- Giardia and Dientamoeba Epidemiology and Disease
- Differentiating Giardia and Dientamoeba Trophozoites from the Commensal Flagellated Trophs
- Differentiating Giardia and Dientamoeba Cysts from those of the Commensal Flagellates
- Other Diagnostic Techniques for Giardia and Dientamoeba
- A physician suspects that a patient may be infected with Giardia and orders an O&P exam. Examination of a trichrome stained smear on the loose sto...
- Match the trophozoite to the image from a trichrome stained smear.
- The best methods for diagnosing G. duodenalis trophozoites include: (choose all that apply)
- Life Cycle, Epidemiology, Disease, and Diagnosis of the Ciliate, Balantidium coli
- Other intestinal Protists: Blastocystis and the Sporozoa (phylum Apicomplexa), and Microsporidia
- Blastocystis spp: Epidemiology, Disease, and Life Cycle
- Blastocystis Diagnosis
- The Intestinal Sporozoa: Cryptosporidium spp., Cyclospora cayetanensis, Cystoisospora belli and Sarcocystis spp.
- Cryptosporidium spp.
- Cyclospora cayetanensis
- Cystoisospora belli (formerly Isospora belli)
- Sarcocystis spp.
- Summary of the Morphologic Features of Blastocystis, Sporozoa and Microsporidia
- The sporozoa are characterized by all of the following except:
- A laboratorian performs an acid-fast stain on a fecal smear and finds several round, acid fast structures measuring 8-10 μm. The most likely diag...
- The organisms belonging to the Microsporidia group have an obligate extracellular phase of development.
Level of Instruction: Intermediate to Advanced
Intended Audience: Medical laboratory scientists, medical laboratory technicians, pathology residents, MLS students, and other health care personnel who have an interest in this subject matter.
Author Information: Margaret Reinhart, MS, MLS(ASCP) is a Senior Lecturer emerita in Biological Sciences at the University of the Sciences in Philadelphia PA where she taught hematology, clinical immunology, parasitology, and other related courses, as well as directed the MLS program for over 30 years. She is currently adjunct instructor in Hematology at Pennsylvania Hospital, Philadelphia PA. She holds a Masters Degree in Biology and in Health Care Administration.
Diane M. Valentin, MS, MT(ASCP)MB is Ms. Valentin is a graduate of University of the Sciences in Philadelphia. Ms. Valentin is a graduate of University of the Sciences in Philadelphia (MT ’99) who has been working as a medical laboratory professional since her graduation. She also has earned a master’s of science degree in Biomedical Informatics, focusing in Bioinformatics, from Rutgers University and a certificate in Molecular Laboratory Diagnostics from Michigan State University. She holds two certifications by the American Society of Clinical Pathology (ASCP): Medical Technologist and Technologist in Molecular Biology.
Rory Huschka, M. Ed., MT(ASCP), has over 20 years of combined experience as a medical technologist, technical supervisor, professor, and manager. He is the former Director of Clinical Laboratory Science and Medical Laboratory Technician Programs at Brookline College. He is currently a Program Director at MediaLab, Inc. Rory holds a BS degree in Medical Technology from North Dakota State University and a Masters in Educational Leadership.