Summary of the Morphologic Features of Blastocystis, Sporozoa and Microsporidia

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The page below is a sample from the LabCE course Intestinal Protozoan Parasites. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Summary of the Morphologic Features of Blastocystis, Sporozoa and Microsporidia

Table 8 provides a brief comparison of the size, shape, and other morphologic features of the "other" protistan parasites seen in the stool, the Microsporidia, the Sporozoa, and Blastocystis covered in this course.
Table 8. Summary of the Morphologic Features of Blastocystis, Sporozoa, and Microsporidia.
OrganismSize and ShapeComments
Cryptosporidium spp. Oocyst round; 4-6 μm Oocyst is diagnostic in the stool; 4 sporozoites occ. seen in oocyst; acid-fast positive
Cyclospora cayetanensis Oocyst round; 8-10 μm Oocyst stained with acid fast stain is variable in color; looks similar to Cryptosporidium, but twice as large
Cystoisospora belliEllipsoidal oocyst; 20-30 μm x 10-19 μm Mature oocyst contains two sporocysts each having 4 sporozoites; acid-fast positive
Sarcocystis spp. Thin-walled oocyst contains 2 ovoid sporocysts which each measure 10-16 μm x 7.5-12 μmOvoid sporocyst seen either together in oocyst or singly; fluorescence under UV light
Blastocystis hominisRound organisms; 6-40 μm; can contain a large central body looking like a vacuole Amoebic form difficult to see; the central body form varies widely in size; can be seen in wet mounts
MicrosporidiaSpores usually round, very small; 0.7-4 μmSpecial stains such as chromotrope (modified trichrome) and chemofluorescent optical brightening agents work best