Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain (Online CE Course)

(based on 90 customer ratings)

Author: Cynthia Sampias, JD, CT(ASCP)HTL
Reviewer: Donna M. Hedger, BS, HT(ASCP)

This course is intended to examine common challenges with H&E staining and offer guidance for troubleshooting.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 10/31/2025
Approved through 10/31/2025


  • Review the basics of H&E staining and what optimal staining should look like.
  • Identify common problems encountered with staining techniques.
  • Consider solutions to common artifacts that influence stain results.
  • Discuss the advantages of staining platforms and the use of kits to standardize stain performance.
  • Address outside factors that can influence H&E stain success.
  • Identify strategies used for successful H&E staining.

Customer Ratings

(based on 90 customer ratings)

Course Outline

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Additional Information

Level of Instruction: Basic to intermediate

Intended Audience: Clinical laboratory histotechnologists, technicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.

Author information: Cynthia Sampias, JD, CT(ASCP)HTL is currently a Senior Field Support Specialist with Leica Biosystems. Her primary focus is to assist histology teams on all core histology products/consumables and troubleshooting. During her career, she worked as a Histotechnologist and Global Project Manager for Covance Central Laboratory Services and as a Senior Cytotechnologist/Histotechnologist at Diagnostic Cytology Laboratories, both located in Indianapolis, Indiana. She holds a Doctor of Jurisprudence degree from Indiana University School of Law with a specialty in health care law, and a Masters of Public Affairs degree in Health Services Administration from Indiana University Northwest. She is certified as both a cytotechnologist and histotechnologist.

Reviewer information: Donna M. Hedger, BS HT(ASCP), is currently a sales specialist at MediaLab. After graduating from Georgia Southern University with a Bachelor of Science in Biology, she spent 25 years working in the operations of the healthcare laboratory at Northside Gwinnett (formerly Gwinnett Medical Center). During her tenure there, she worked in various roles, including phlebotomy, lab assistant, pathology assistant, histology technician and more recently as the manager of the Pathology Laboratory for 18 years. She is a certified histology technician.

Course description: This course is intended to examine common challenges with H&E staining and offer guidance for troubleshooting.

Automated H&E Stainer


Sebaceous gland showing pyknotic nuclei, visible on top half of the image.

drying and cracking

Good diff in eosin

Automated staining instrument with interface capability

Good staining - kidney