False-Negative Reactions with Rh Typing Reagents

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The page below is a sample from the LabCE course Overview Of Major Antigens of the Rh Blood Group System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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False-Negative Reactions with Rh Typing Reagents

Table 7 below delineates some of the more commonly seen false-negative reactions with Rh typing reagents and provides insight into the causes and corrective actions that may be undertaken when these problems are presented.
Table 7. False-Negative Reactions with Rh Typing Reagents.
Likely CauseCorrective Action
Failure to follow manufacturer's instructions preciselyReview direction circular; repeat test
Resuspension too vigorousResuspend all tubes gently during repeat testing
Immunoglobulin-coated cells (in vivo)Use saline-active typing reagent; repeat test
Incorrect reagent selectedRead vial carefully; repeat test
Reagent deteriorationOpen new vial, perform appropriate QC; repeat test