Paracoccidioides brasiliensis

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The page below is a sample from the LabCE course Fungal Infections in Humans. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Paracoccidioides brasiliensis

Paracoccidioides brasiliensis produces large, round yeast cells (10-50 µm) with circumferential budding, giving the appearance of a mariner's wheel in tissue sections or primary wet preparations. The daughter cells bud on a narrow base from the mother cell.
The top right image shows a lactophenol cotton blue preparation of P. brasiliensis demonstrating budding yeast cells. The bottom right image shows a methenamine silver stain of P. brasiliensis demonstrating the classic "mariner's wheel"—buds projecting from the yeast cell.
51. CDC/Georg. Image #4296. Under a magnification of 970X, this photomicrograph depicts budding cells of the fungus organism, Paracoccidioides brasiliensis, during its yeast phase. PHIL public domain. Created 1963. Accessed January 15, 2023.
52. CDC/Georg. Image #527. This photomicrograph of a methenamine silver-stained tissue sample revealed some of the histopathology exhibited in a case of paracoccidioidomycosis. Note the multiple buds projecting from one of the Paracoccidioides brasiliensis yeast form cells. PHIL public domain. Created 1963. Accessed January 15, 2023.

51. Budding yeast cells are seen on a lactophenol cotton blue prep of Paracoccidioides.
52. Gomori methenamine silver (GMS) stained tissue sample showing Paracoccidioides brasiliensis "mariner's wheel".