Course Outline
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- Medically important fungi
- Yeast
- Yeast
- Cryptococcus - Introduction
- Cryptococcus Around the World
- Cryptococcal Infections
- Cryptococcus - Identification
- What is a useful stain for the identification of Cryptococcus?
- Cryptococci are commonly found in soil contaminated by bird droppings and in decaying wood and in tree hollows.
- Candida species
- Candida glabrata
- Which features distinguishes Candida glabrata from other infectious Candida species?
- Rhodotorula
- Rhodotorula spp. are yeasts that produce a pyocyanin pigment ranging from yellowish to red that can be visualized with individual colonies.
- Malassezia
- Which supplement is necessary to add to culture media to ensure proper growth of Malassezia species?
- Saccharomyces cerevisiae
- Trichosporon
- Trichosporon, continued
- Trichosporon yeast forms arthroconidia and hyphal elements are larger than those of Candida spp.
- Blastoschizomyces (Geotrichum)
- Molds
- Aspergillus
- Aspergillus
- Aspergillus, continued
- Aspergillus niger
- What is a distinguishing feature of Aspergillus spp. in tissue identification?
- Fusarium
- Fusarium
- What shape is commonly associated with the description of the macroconidia of Fusarium spp.?
- Dermatophytes
- Dermatophytes (Epidermophyton, Microsporum, Trichophyton) - Introduction
- Which enzymes do dermatophytes employ to digest hair, nails, and the stratum corneum of skin?
- Dermatophytes (Epidermophyton, Microsporum, Trichophyton)
- Epidermophyton floccosum always produces microconidia.
- Which antibiotic is used in growth media to help isolate dermatophytes?
- Dermatophytes (Epidermophyton, Microsporum, Trichophyton)
- Dermatophytes (Epidermophyton, Microsporum, Trichophyton)
- Dermatophytes (Epidermophyton, Microsporum, Trichophyton)
- Dermatophytes (Epidermophyton, Microsporum, Trichophyton)
- Dematiaceous Molds
- Dematiaceous molds - Introduction
- Dematiaceous molds are darkly pigmented on both the surface and reverse side of the culture plate.
- Dematiaceous Molds, continued
- Which is a feature that can differentiate a rapidly growing dematiaceous mold from a slowly growing dematiaceous mold?
- Dematiaceous molds - Alternaria
- Dematiaceous molds - Scedosporium
- Lomentospora prolificans has a gray or black surface and reverse.
- Mucormycetes
- Mucormycetes (Zygomycetes) - Introduction
- If zygomycosis is clinically suspected, then how should the specimen be prepared prior to culture?
- Mucormycetes, continued
- The Mucor spp. do not produce rhizoids.
- Dimorphic Fungi
- Dimorphic Fungi
- Dimorphic Fungi - Introduction
- How does one confirm that a fungus is thermally dimorphic?
- Dimorphic fungi-Coccidioides spp.
- Arthroconidia of Coccidioides spp. are oblong shaped and alternate with empty cells.
- Dimorphic fungi-Coccidioides spp.
- In addition to culture, diagnosis of coccidioidomycosis can be accomplished by which of the following?
- Dimorphic fungi - Paracoccidioides brasiliensis
- Paracoccidioides brasiliensis gives the appearance of a mariner's wheel in tissues sections or primary wet preparations.
- Dimorphic fungi-Paracoccidioides brasiliensis
- Dimorphic fungi-Blastomyces spp.
- Dimorphic fungi-Blastomyces spp., continued
- What is the characteristic budding morphology that can help identify Blastomyces spp.?
- Diagnosis of blastomycosis can also be made with an antigen test performed on urine or serum.
- Dimorphic fungi- Histoplasma spp.
- Which cells are H. capsulatum yeast cells often found within?
- Dimorphic fungi - Histoplasmosis
- Dimorphic fungi - Sporothrix schenckii
- Microscopically, S. schenckii mold-forms produce very delicate hyaline septate hyphae producing conidiophores topped by clusters of macroconidia ("ros...
- Dimorphic fungi - Talaromyces marneffei
- How do T. marneffei yeast-forms replicate?
- References
Additional Information
Level of instruction: Basic to intermediate
Intended audience: This course is intended for microbiology bench technicians and technologists, supervisors, and administrators.
Course Description: Upon completion of the course, the reader will have accomplished several skills: recognize the geographic distribution and anatomical predilection of the primary fungi causing human disease, recognize the clinical manifestation of disease, and identify pathogenic fungi through their unique distinguishing features such as culture growth characteristics and morphology.
Author information: Timothy Walls, M.D., M.S., is an AP/CP boarded pathologist. He has completed fellowships in medical microbiology as well as molecular genetic pathology. Currently he is the Director of Clinical Laboratories at Sentara Reference Laboratories. Timothy has earned his B.S. in Biochemistry, Cellular and Molecular Biology at the University of Tennessee, his M.S. in Forensic Science at Drexel University and his M.D. from the University of Tennessee Health Science Center.
Reviewer information: Ryan Relich, PhD, D(ABMM), MLS(ASCP)SM is a medical microbiologist. He serves as the interim Medical Director of the Indiana University (IU) Health Division of Clinical Microbiology, Medical Director of the IU Health Special Pathogens Unit Laboratory, Associate Medical Director of the IU Health Division of Molecular Pathology, and Section Director of Clinical Microbiology and Serology Laboratories for Eskenazi Health. Dr. Relich is an Assistant Professor of Clinical Pathology and Laboratory Medicine at IU School of Medicine, and conducts both applied and translational research in the areas of diagnostic test development and evaluation; novel and emerging virus ecology, epidemiology, and pathogenesis; and pandemic preparedness. Dr. Relich holds a PhD in microbiology (Miami University), a BS in medical technology (Clarion University of Pennsylvania), and a BS in molecular biology and biotechnology (Clarion University of Pennsylvania), as well as certifications through the American Board of Medical Microbiology and American Society for Clinical Pathology.