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The page below is a sample from the LabCE course Laboratory Diagnosis of Cystic Fibrosis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Laboratory Diagnosis of Cystic Fibrosis (online CE course)

CFF. "Sweat Test." Cystic Fibrosis Foundation.

Clinical and Laboratory Standards Institute (CLSI). Newborn Screening for Cystic Fibrosis; Approved Guideline. CLSI document NBS05-A (formerly I/LA35-A). Wayne, PA: CLSI; 2011.
Clinical and Laboratory Standards Institute (CLSI). Sweat Testing: Sample Collection and Quantitative Chloride Analysis; Approved Guideline. CLSI document C34-A3. Wayne, PA: CLSI; 2009.
Cystic Fibrosis Foundation Patient Registry. Annual Report 2021. Bethesda MD. Available at
Farrell PM, White TB, et al. Diagnosis of Cystic Fibrosis: Consensus Guidelines from the Cystic Fibrosis Foundation. The Journal of Pediatrics. 2017 181(1): S4-15.
Gibson LE, Cooke RE: A test for concentration of electrolytes in sweat in cystic fibrosis of the pancreas utilizing pilocarpine by iontophoresis. Pediatrics. 1959;23:731-737.
Katkin JP. Cystic Fibrosis: Clinical manifestations and diagnosis. UpToDate website. Last updated May 2, 2022. Available at:
LeGrys VA, McColley SA, Li Z, Farrell PM. "The need for quality improvement in sweat testing infants after newborn screening for cystic fibrosis." The Journal of Pediatrics. 2010 Dec 16;157(6):1035-7.
LeGrys VA, Yankaskas JR, Quittell LM, Marshall BC, Mogayzel PJ Jr. Diagnostic sweat testing: the Cystic Fibrosis Foundation guidelines. The Journal of Pediatrics. 2007 Jul;151(1):85-9.
Littlewood J. The history of cystic fibrosis.
NIH. "Cystic Fibrosis Symptoms." National Heart, Lung, and Blood Institute. March 2022,
Simon, RH. Cystic Fibrosis: Antibiotic therapy for lung disease. UpToDate website. Last updated August 16, 2021. Available at:
Technical Standards and Guidelines for CFTR Mutation Testing: 2020 Edition. American College of Medical Genetics. Available at:
The UK Cystic Fibrosis Gene Therapy Consortium