Current and Future Prospects

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The page below is a sample from the LabCE course Molecular Methods in Clinical Microbiology (A Historical Review). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Current and Future Prospects

Cepheid's next-generation platform, the Xpert® system, addressed some of the problematic factors by simplifying the workflow and making on-demand testing feasible. The assay targets the SCCmec-orfX junction and utilizes a self-contained cartridge containing all the reagents necessary for real-time PCR. Swabs are placed into an elution fluid and vortexed; an aliquot is dispensed into a reagent cartridge, which is installed on the Xpert platform. Results are available in approximately 75 minutes. Capital investment for equipment and ongoing reagent costs remain a consideration with all system platforms.
Another consideration for laboratories is throughput. The system contains independent modules for testing, and the user is able to use a small platform (up to 16 modules) or a large platform (a single Infinity-80, configured with up to 80 modules), depending on need. Facilities that need to process very large volumes of specimens will require multiple modules, adding to capital considerations.
Large volume platforms that automate sample preparation, extraction, amplification, and detection in a real-time format would represent the ideal, walk-away solution for many larger laboratories, as well as a target for development for many manufacturers.