Differentiation between Myeloid and Lymphoblastic Leukemias Using Cluster of Differentiation (CD) Markers

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The page below is a sample from the LabCE course Acute Leukemia with a Focus on WHO Classification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Differentiation between Myeloid and Lymphoblastic Leukemias Using Cluster of Differentiation (CD) Markers

As previously mentioned, other tests exist to differentiate between myeloid and lymphoid lines. One way is by the use of monoclonal antibodies to detect surface markers on the cells. This is done using flow cytometry techniques which will be described in more detail later. As far as initial differentiation, however, Table 4 is useful to differentiate the major cell lines.
Table 4. Differentiation between Myeloid and Lymphoblastic Leukemias Using CD Markers.
Cell LineageCD Markers
Myeloid CD13, CD33, CD15, CD117
T-Cell CD2, CD3, CD4, CD5, CD7, CD8
B-Cell CD19, CD20, CD22, CD79a
Megakaryoblastic CD41, CD61