Holzer's Crystal Violet Staining - Chemistry

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Holzer's Crystal Violet Staining - Chemistry

Holzer's crystal violet stain is used to identify glial cells and processes. Glial cells that have been stained with crystal violet are resistant to decolorization due to the alkalinity of the aniline-chloroform mixture.

Glial cells stained with Holzer's crystal violet. Image courtesy of Wikimedia Commons.