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Histology Special Stains: Nervous Tissue (Online CE Course)

(based on 97 customer ratings)

Author: Tanisha N. Neely, HT(ASCP)
Reviewer: Diana Harrington, BS, HT(ASCP)

This is the final course in the series that will explore special stains used in the clinical histology laboratory. Basic nervous tissue staining mechanisms and classification of nervous tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate nervous tissue in samples.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-053-16, approved through 7/31/2018
Course number 20-547703, approved through 9/1/2018

Objectives

  • Understand basic tissue staining methods used in the clinical histology laboratory.
  • Explain factors that affect dye binding and use to troubleshoot staining issues.
  • Classify nervous tissue elements and identify which dyes will bind to these elements.
  • Demonstrate safe microwave use to perform special staining procedures.

Customer Ratings

(based on 97 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Introduction
      • Tissue Staining in the Clinical Histology Laboratory
      • The Biological Stain Commission (BSC)
      • Cellular and intracellular structure of tissue samples cannot be microscopically examined until they are colored by dyes.
      • What are dyes called that are derived through chemical reactions?
  • Basic Staining Mechanisms
      • What Makes a Dye?
      • Factors that Affect Dye Binding
      • Acids, Bases, pH, and Buffers
      • Basophilic and Acidophilic Staining
      • Oxidation and Reduction
      • Acidic dyes contain basic groups that have an affinity for acidic tissue elements.
      • What is the chemical reaction called that results in the removal of electrons from a molecule?
  • Nervous System Overview
      • Classifying Nervous Tissue
      • Functions of Nervous Tissue
      • Neurons: A Visual Overview
      • Histology of the Nervous System: Neurons
      • Histology of the Nervous System: Neurofibrillary Tangles (NFTs)
      • Histology of the Nervous System: Nissl
      • Histology of the Nervous System: Neuroglia
      • Histology of the Nervous System: Myelin
      • The central nervous system (CNS) consists of the brain and spinal cord.
      • Cell processes arising from the neuron cell body consist of which of the following?
  • Special Staining Methods Used to Demonstrate Nervous Tissue
  • Using the Microwave for Special Staining Procedures
  • References
      • References

Additional Information

Level of instruction: Intermediate
 
Intended audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.  
 
Author information: Tanisha N. Neely, HT(ASCP) is a freelance writer with a work history in histology, microbiology, and pharmaceutical research. Ms. Neely attended Indiana University where she earned a bachelors degree in arts & humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
 
Reviewer information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
 
Course description: This is the final course in the series that will explore special stains used in the clinical histology laboratory. Basic nervous tissue staining mechanisms and classification of nervous tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate nervous tissue in samples. 

Bielchowsky stained cerebellum. Image courtesy of Wikimedia Commons.
Glial cells in a rat brain, courtesy Wikimedia Commons.
Luxol fast blue
Neuron and Nissl stained with Cresyl Echt Violet.
pH range
How to Subscribe
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Individual course$20 Add to cart


Bielchowsky stained cerebellum. Image courtesy of Wikimedia Commons.


Glial cells in a rat brain, courtesy Wikimedia Commons.


Luxol fast blue


Neuron and Nissl stained with Cresyl Echt Violet.


pH range