Histology Special Stains: Nervous Tissue (Online CE Course)

(based on 131 customer ratings)

Author: Tanisha N. Neely, HT(ASCP)
Reviewers: Diana Harrington, BS, HT(ASCP) and Carla Shoffeitt, MSM, HT(ASCP)

This is the final course in the series that will explore special stains used in the clinical histology laboratory. Basic nervous tissue staining mechanisms and classification of nervous tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate nervous tissue in samples.

See all available courses

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 11/30/2024
Approved through 11/30/2024

Objectives

  • Explain basic tissue staining methods used in the clinical histology laboratory.
  • Explain factors that affect dye binding and use to troubleshoot staining issues.
  • Classify nervous tissue elements and identify which dyes will bind to these elements.
  • Demonstrate safe microwave use to perform special staining procedures.

Customer Ratings

(based on 131 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.

Additional Information

Level of Instruction: Intermediate
Intended Audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and medical laboratory science students, pathology residents, and practicing pathologists.  
Author Information: Tanisha N. Neely, HT(ASCP), is a freelance writer with work history in histology, microbiology, and pharmaceutical research. She attended Indiana University, where she earned a bachelor's degree in arts and humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
Reviewer Information:
Diana Harrington, BS, HT(ASCP), is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
Carla J. Shoffeitt, MSM, HT(ASCP), is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology. 
Course Description: This is the final course in the series that will explore special stains used in the clinical histology laboratory. Basic nervous tissue staining mechanisms and classification of nervous tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate nervous tissue in samples. 

Bielchowsky stained cerebellum. Image courtesy of Wikimedia Commons.


Glial cells in a rat brain, courtesy Wikimedia Commons.


Luxol fast blue


Neuron and Nissl stained with Cresyl Echt Violet.


pH range