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The page below is a sample from the LabCE course Hemoglobinopathies: Hemoglobin S Disorders. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Hemoglobin Electrophoresis

The lanes labeled as "patient 3*" correlate with this case of double heterozygous Hb SC. Notice that the tracing indicates 0.9% Hb F. The actual amount of Hb F should be determined using high performance liquid chromatography (HPLC).
The attached PDF can be used as a key to determine the areas of migration.

Migration of Hemoglobin in Alkaline_Acid Electrophoresis.pdf [click to view / print]

Adobe Acrobat PDF file