Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Hemoglobinopathies: Hemoglobin S Disorders. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Hemoglobinopathies: Hemoglobin S Disorders (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 129 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Migration of Hemoglobin in Acid Electrophoresis

Acid electrophoresis (pH 6.0) is used to confirm or rule out the presence of hemoglobins D and G that migrate with hemoglobin S in alkaline electrophoresis (pH 8.6). Hb A and F migrate from the center-point of application toward the cathode, with Hb F migrating the furthest.

Relative migrations of hemoglobin variants on acid electrophoresis can be seen below. The application point is indicated by the arrow. In this image, hemoglobin D is now separated out from hemoglobin S. However, because it migrates with Hb A in acid electrophoresis, further testing would be needed to identify Hb D.