Migration of Hemoglobin in Acid Electrophoresis

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The page below is a sample from the LabCE course Hemoglobinopathies: Hemoglobin S Disorders. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Migration of Hemoglobin in Acid Electrophoresis

Acid electrophoresis (pH 6.0) is used to confirm or rule out the presence of hemoglobins D and G that migrate with hemoglobin S in alkaline electrophoresis (pH 8.6). Hb A and F migrate from the center-point of application toward the cathode, with Hb F migrating the furthest.

Relative migrations of hemoglobin variants on acid electrophoresis can be seen below. The application point is indicated by the arrow. In this image, hemoglobin D is now separated out from hemoglobin S. However, because it migrates with Hb A in acid electrophoresis, further testing would be needed to identify Hb D.