Distinguishing Between Red and White Blood Cells

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The page below is a sample from the LabCE course The Urine Microscopic: Microscopic Analysis of Urine Sediment. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about The Urine Microscopic: Microscopic Analysis of Urine Sediment (online CE course)
Distinguishing Between Red and White Blood Cells

Many blood cells are visible in urine sediments, as observed in the top image. When differentiating RBCs from WBCs, it is important to use the fine focus adjustment on the microscope. WBCs have a nucleus that will be visible with the fine focus, whereas RBCs do not.

Supravital stain can also help to distinguish RBCs from WBCs. Two RBCs are indicated by blue arrows, and a WBC is indicated by the red arrow in the lower image of supravital-stained sediment.