The Urine Microscopic: Microscopic Analysis of Urine Sediment (Online CE Course)

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 3 hour(s) Approved through 9/30/2024

Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 3 hour(s) Approved through 9/30/2024

Objectives

• Explain the importance of the microscopic examination.
• Describe the correct preparation of the urine sediment.
• Recognize cells, casts, bacteria, yeast, crystals, and other structures that may be present in urine sediment.
• Recognize artifacts in urine sediment and distinguish from significant findings.
• Correlate microscopic findings with urine reagent strip findings.

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Course Outline

• Introduction to the Urine Microscopic
• Course Introduction
• Specimen Collection and Preparation
• Urine Specimen Collection
• Specimen Collection and Storage
• Steps in Preparing a Concentrated Urine Sediment
• Microscopic Examination of Urine Sediment
• The volume of urine that is centrifuged and the amount of sediment that is used for microscopic examination should be the same for all persons perform...
• Casts
• Formation and Significance of Casts
• Factors Promoting Cast Formation
• Hyaline Casts
• Hyaline Casts: High Power
• Hyaline Casts: Phase-Contrast Microscopy
• Cellular Casts
• White Cell Casts
• Red Cell Casts
• Renal Tubular Epithelial (RTE) Cell Casts
• Granular Casts
• Waxy Casts
• Fatty Casts
• Broad Casts
• Table: Clinical Significance of Casts
• Which of the following are characteristics of casts? (Choose all that apply.)
• All of the following factors promote cast formation except:
• Match the following descriptions with the casts they describe.
• Cellular Elements
• Cell Types Observed in Urine Sediment
• Squamous Epithelial Cells
• Squamous Epithelial Cells: Phase-Contrast Microscopy
• Transitional Epithelial Cells
• Renal Tubular Epithelial (RTE) Cells
• White Blood Cells (WBCs)
• WBC Clumps
• Red Blood Cells (RBCs)
• Crenated Red Cells
• Ghost Cells
• Distinguishing Between Red and White Cells
• Characteristics of Cellular Elements
• Which of the following are characteristic of normal RBCs when viewed microscopically?
• All of the following are characteristic of WBCs under high power (400X magnification) brightfield microscopy except that they:
• Bacteria, Yeast, and Parasites
• Yeast
• Bacteria
• Parasites
• Trichomonas: Phase-Contrast Microscopy
• Enterobius vermicularis: Phase-Contrast Microscopy
• Match the following:
• Which of the following helps to distinguish yeast from red blood cells (RBCs) when performing a microscopic examination of urine sediment?
• Common Crystals
• Crystals
• Identification of Crystals
• Table: Normal Crystals
• Which of the following may be found in normal acidic urine? (Choose all that apply.)
• Which of the following may be found in normal alkaline urine? (Choose all that apply.)
• Match each of the crystals shown below that may be seen in normal urine with its identification from the drop-down box.
• Abnormal Crystals
• Abnormal Crystals
• Crystals of Clinical Significance
• Leucine Crystals
• Tyrosine Crystals
• Cystine Crystals
• Cholesterol Crystals
• Bilirubin Crystals
• Summary of Abnormal Crystals
• Which of the following abnormal crystals may indicate liver disease?
• Match the following:
• True or False: This slide shows tyrosine crystals.
• True or False: This slide shows leucine crystals.
• True or False: This slide shows cholesterol crystals.
• True or False: This slide shows leucine crystals.
• True or False: This slide shows cholesterol crystals.
• Artifacts in Urine Sediment
• Introduction to Artifacts in Urine Sediment
• Oil or Fat Droplets
• Air Bubbles
• Starch Granules
• Fibers
• True or False: The structure indicated by the arrow is a fiber and would not be reported.
• Miscellaneous Elements
• Sperm
• Mucous Threads
• Mucous Threads: Phase-Contrast Microscopy
• True or False: Hyaline casts are present in this urine sediment microscopic field.
• Distinguishing Structures That May Be Confused With Each Other
• Introduction to Distinguishing Similar Elements
• Distinguishing Yeast from Red Blood Cells
• Distinguishing Renal Tubular Epithelial (RTE) Cells from White Blood Cells (WBCs)
• Bacteria and Amorphous Material
• Fat Droplets and RBCs
• Starch Granules and Urinary Crystals
• Distinguishing Eosinophils from Neutrophils
• Iodine will confirm the presence of:
• A urine specimen was delayed in transport to a medical laboratory. Upon examination of the urine sediment, the medical laboratory scientist cannot dis...
• A medical laboratory scientist is trying to enumerate red blood cells in sediment containing numerous fat droplets. Which of the following reagents wo...
• Correlation of Microscopic and Macroscopic Results
• Correlation of Results
• References
• References

Additional Information