Cold Autoantibody Example

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Cold Autoantibody Example

Reactions are occurring strongly in IS phase but are weaker in AHG phase, which could be due to some disassociation of the cold antibody occurring at the warmer testing phase.
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+3+W+NT
2++00++++0+0+++0+0+0++++0+3+W+NT
3+0++000+0+0+0+0+0++00++0+3+W+NT
4+0+0+00+0+0+00+00++++0+0+3+W+NT
50++0+00+0+0++++++00+0+00+3+W+NT
600+++0++0+0+0+++0+++++00+3+W+NT
700+0+00+0+0++00++0+00++0+3+W+NT
800+0+00+0+0+++0+0+++++00+3+W+NT
900+0+00+0+0++++00+0++0+0+3+W+NT
10+0+0+0++0+0+0+0+0+0++0+0+3+W+NT
AC3+W+NT
Prewarming of all reagents and sample will prevent binding of cold antibody. If the W+ reactions at AHG are due to residual cold antibody, the reactions should be negative with a prewarmed panel. No IS phase reading is performed.
Prewarmed Panel
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+NT0
2++00++++0+0+++0+0+0++++0+NT0
3+0++000+0+0+0+0+0++00++0+NT0
4+0+0+00+0+0+00+00++++0+0+NT0
50++0+00+0+0++++++00+0+00+NT0
600+++0++0+0+0+++0+++++00+NT0
700+0+00+0+0++00++0+00++0+NT0
800+0+00+0+0+++0+0+++++00+NT0
900+0+00+0+0++++00+0++0+0+NT0
10+0+0+0++0+0+0+0+0+0++0+0+NT0
ACNT0