Example of Cold Autoantibody

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example of Cold Autoantibody

Reactions are occurring strongly in the IS phase but are weaker in the AHG phase, which could be due to some disassociation of the cold antibody occurring at the warmer testing phase.
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+3+W+NT
2++00++++0+0+++0+0+0++++0+3+W+NT
3+0++000+0+0+0+0+0++00++0+3+W+NT
4+0+0+00+0+0+00+00++++0+0+3+W+NT
50++0+00+0+0++++++00+0+00+3+W+NT
600+++0++0+0+0+++0+++++00+3+W+NT
700+0+00+0+0++00++0+00++0+3+W+NT
800+0+00+0+0+++0+0+++++00+3+W+NT
900+0+00+0+0++++00+0++0+0+3+W+NT
10+0+0+0++0+0+0+0+0+0++0+0+3+W+NT
AC3+W+NT
Prewarming of all reagents and sample will prevent the binding of the cold antibody. If the W+ reactions at AHG are due to residual cold antibody, the reactions should be negative with a prewarmed panel. No IS phase reading is performed.
Prewarmed Panel
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+NT02+
2++00++++0+0+++0+0+0++++0+NT02+
3+0++000+0+0+0+0+0++00++0+NT02+
4+0+0+00+0+0+00+00++++0+0+NT02+
50++0+00+0+0++++++00+0+00+NT02+
600+++0++0+0+0+++0+++++00+NT02+
700+0+00+0+0++00++0+00++0+NT02+
800+0+00+0+0+++0+0+++++00+NT02+
900+0+00+0+0++++00+0++0+0+NT02+
10+0+0+0++0+0+0+0+0+0++0+0+NT02+
ACNT02+