Example of Multiple Antibodies, continued: Selected Cell Rule-Out Panel

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example of Multiple Antibodies, continued: Selected Cell Rule-Out Panel

Selected cells were chosen that are D-negative because anti-D is strongly suspected. Remember in the original panel that C, E, K, Fyb, Jka, Leb, and S could not be ruled out. The results are below.
  • Cell #1 is homozygous positive for C, K, and Leb
  • Cell #3 is homozygous positive for E, Fyb, Jka, and Leb
  • Cell #4 is homozygous positive for C, E, and K
  • Cell #6 is homozygous positive for E, Fyb, Jka, and Leb
  • Cells #2 and 5 are positive for S and are reacting with the patient serum
Rh-HrKellDuffyKiddLewisMNSsP1Lutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubIS37AHGCC
10+00+0+00+0++00+0++00++0+002+
20++0+00+0+0+0+0++0+++0+0+03+NT
300++000+0+0+0++00++00++0+002+
40+0+00+00+0++00++00+0++0+002+
500+0+00+0+0++++00++0+0+0+03+NT
600++00++0+0+0++00+0+0++0+002+
Rule-outs are highlighted in light teal.
The second antibody identified based on panel cells 2 and 5 is anti-S. The patient has anti-D and anti-S. Refer to the following page for an explanation of the varying strengths of S.