Example of Dosage and/or Multiple Antibodies Influencing the Strength of Reactions

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example of Dosage and/or Multiple Antibodies Influencing the Strength of Reactions

Varying reaction strengths in the same phase could indicate antibody showing dosage, multiple antibodies, or both.
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+04+NT
2++00++++0+0+++0+0+0++++0+03+NT
3+0++000+0+0+0+0+0++00++0+002+
4+0+0+00+0+0+00+00++++0+0+04+NT
50++0+00+0+0++++++00+0+00+02+NT
600+++0++0+0+0++00+++0+00+03+NT
700+0+00+0+0++00++0+0+0+0+04+NT
800+0+00+0+0+++0+0+++++00+03+NT
900+0+00+0+0++++00+0++0+0+04+NT
10+0+0+0++0+0+0+0+0+0++0+0+04+NT
AC 002+
IS = Immediate Spin; AHG = Antihuman Globulin Phase; CC = Check Cells; NT = Not Tested; AC = Auto Control
Jka and S are the antibodies that are present. Weaker reactions can be seen when either of the target antigens is present alone and/or in the heterozygous state on the cell.
4+ reaction in panel cell 1, 4 and 9: Both Jka and S are present
4+ reaction in panel cell 7 and 10: S present (homozygous)
3+ reaction in panel cell 6: Jka present (homozygous)
3+ reaction in panel cells 2 and 8: S present (heterozygous)
2+ reaction in panel cell 5: Jka present (heterozygous)