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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Case Study 1: Immune Alloantibody

A 42-year-old male received six units of Red Blood Cells during open heart surgery six months ago. His antibody screen was negative at that time. He has returned for a follow up surgery and his antibody screen is now positive with both screen cells at the AHG phase.
(IS = Immediate Spin; AHG = Antihuman Globulin Phase; CC = Check Cells; AC = Auto Control; ND= Not done)

Refer to Case Study 1 panel below to see reactions of antibody panel.
  • Reactions are occurring at the AHG phase. This indicates an IgG clinically significant antibody.
  • Reactions are multiple strengths indicating either multiple antibodies or a single antibody showing dosage.
  • The autocontrol is negative which indicates the patient has an alloantibody.