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The page below is a sample from the LabCE course Introduction to Flow Cytometry: Blood Cell Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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CD19 and CD20

These images represent fluorescent monoclonal antibody data for CD19 and CD20 respectively. In each histogram, the peak to the left of the vertical line represents cells that are negative for the marker, and the peak to the right of the vertical line represents cells that are positive for the marker. This means that the percentage of cells in the gated population (lymphocytes) that have CD19 cell surface antigens is 6.8% and CD20 positive cells comprise 6.7% of the gated population.
A CD5 analysis further confirmed that the B-cell population was CD5-negative.
The B-cell expression at this point is:
CD5=0%
CD19= 7%
CD20= 7%