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The page below is a sample from the LabCE course Introduction to Flow Cytometry: Blood Cell Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Using Cell Surface Markers as Tools For Diagnosis

One of the most widely used marker combinations for chronic (mature) lymphocyte analysis is CD5 with CD19. Usually, CD5 is a T cell marker and CD19 is a B cell marker. Normal B cells do not express CD5, but do express CD19. However, abnormal B cells (neoplastic B cells) can express CD5. Therefore, if the cell population expresses both markers, it is highly likely that the patient has a B cell neoplasm, such as chronic lymphoid leukemia (CLL) or mantle cell lymphoma.