Microscopic Morphologic Features of Yeast

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The page below is a sample from the LabCE course Mycology: Yeasts and Dimorphic Pathogens. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Microscopic Morphologic Features of Yeast

The cells shown in Image D are arthroconidia, some showing buds coming off of adjacent corners. This feature is diagnostic for Trichosporon species.
The yeast cells of Cryptococcus neoformans tend to be spherical, irregular in size, produce a single bud attached by a hair-like connector, and are surrounded by polysaccharide capsules, as illustrated in Image E.
Candida species, including C. albicans, produce single buds that tend to elongate, forming the precursor of what will be a pseudohypha. Thus, the cell shown in Image F can be suspected of being a Candida species because of the distinctly elongated bud.
The cells shown in Image G are regular in size, have a spherical bud attached by a connector wider than seen in C. neoformans, and have no surrounding capsular material. This yeast cell is most consistent with Candida (formerly Torulopsis) glabrata.