Mycology: Yeasts and Dimorphic Pathogens (Online CE Course)

(based on 403 customer ratings)

Authors: Elmer W. Koneman, MD
Margaret Reinhart MS, MT(ASCP)
Reviewer: Julie Ann West PhD, MLS (ASCP), SM(ASCP)

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Mycology: Yeasts and Dimorphic Pathogens uses an interrogative, question-and-answer, approach to direct and instruct the participant in identifying yeasts and dimorphic fungi. Numerous images enhance the descriptions of identifying characteristics by illustrating the appearance of fungal colonies on media and microscopic appearance. The course also identifies sources of infection and associated diseases. This course is appropriate for laboratory professionals and students in clinical laboratory science programs who want to increase their understanding of mycology including microscopic and colony appearance and isolate identification.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)
Approved through 4/30/2024
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Microbiology/Mycology/Parasitology): 2 hour(s)
Approved through 9/1/2022

Objectives

  • Define terms that are used to describe morphologic features of molds.
  • Describe preliminary identification methods for various yeasts.
  • Identify yeast species by observing the appearance of the colony morphology and through microscopic appearance.
  • Interpret various yeast patterns of growth on cornmeal agar.
  • Discuss various diseases that are associated with yeasts.
  • Define "dimorphic fungi" and identify dimorphic pathogens.
  • Describe microscopic and colony morphologies that aid in the identification of dimorphic pathogens.
  • Identify sources and diseases that are associated with dimorphic pathogens.
  • Recognize saprophytic molds that can mimic morphologic features of dimorphic pathogens and describe methods to inhibit the growth of these saprophytic counterparts.

Customer Ratings

(based on 403 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • General Information Related to Identification of Molds
  • Morphologic Features of Yeast
      • Preliminary Identification Methods for Various Yeasts
      • Morphologic Features of Yeast Colonies
      • Microscopic Morphologic Features of Yeast
      • Malassezia
      • Which of the organisms listed below has the unique property of producing germ tubes when fetal bovine serum is lightly inoculated with the yeast isola...
      • The growth of the yeast-like colonies shown in the upper image was obtained on blood agar from a skin culture only in the area overlaid by virgin oliv...
  • Yeast Identification Using Cornmeal Agar
      • Interpretation of Growth Patterns on Cornmeal Agar
      • Examples of Cornmeal Agar Morphologies
      • Match each of the cornmeal agar morphologies listed in the drop-down box with the name of its corresponding yeast species on the right.
      • Match each of the fungal species listed below with the appropriate category, indicating whether or not it has the capability of producing pseudohyphae...
      • A yeast identification system gave a biotype number for an unknown isolate that did not differentiate between Candida tropicalis and Candida parapsilo...
  • Extending the Preliminary Identification to Full Identification
      • Evaluating the Need for Full Identification of Yeast
      • Recommended Procedure for Identification of Cryptococcus neoformans
      • Arrange in sequence the steps that should be taken to make a definitive identification of Cryptococcus neoformans.
      • The colonies illustrated in this photograph were recovered from a blood culture after 48 hour incubation at 30°C. What is the MOST likely source ...
      • Illustrated in this photomicrograph of a lactophenol blue preparation of a urine sediment is a cluster of yeast cells that were presumptively identifi...
  • Dimorphic Pathogens
      • What are Dimorphic Fungi
      • Microscopic Morphologic Features of the Mold Forms of Various Dimorphic Fungi
      • Microscopic Morphologic Features of the Yeast Forms of Various Dimorphic Fungi
      • Culture Confirmation Methods
      • Histoplasma capsulatum
      • Coccidioides immitis
      • Sporothrix schenckii
      • Blastomyces dermatitidis
      • Paracoccidioides brasiliensis
      • Match each of the names of the animals with the names of the dimorphic fungi listed that most commonly may be related to transmission of disease to hu...
      • The colonies shown in the upper image were obtained on blood agar from a sputum specimen after 10 days incubation at 30°C. The lower image is a l...
      • The growth of the colonies shown in the upper image was obtained on blood agar from a sputum specimen after incubation at 30°C. The lower image i...
      • The colonies shown in the upper image, obtained from a biopsy of an ulcerating skin lesion of the arm, are growing on agar slants of Sabouraud's dextr...
      • The upper image illustrates a subculture of a mold colony suspected of being a dimorphic fungus inoculated to the surface of blood agar and incubated ...
      • Although care should be taken when working with all fungus cultures in the laboratory, personnel are particularly prone to develop laboratory acquired...
      • Which dimorphic fungus may produce black, yeast-like colonies after prolonged incubation at 37°C?
      • Match each of the names of the dimorphic fungal species shown with its mold form the drop-down box.
      • The images below are lactophenol blue mounts of the yeast forms of various dimorphic fungi. Which of the images is consistent with an identification o...
  • Differentiating Dimorphic Molds from Saprophytic Counterparts
  • References
      • References

Additional Information

Level of instruction: Intermediate
 
Target audience: Microbiologists, medical laboratory scientists, medical laboratory technicians, and MLS students.
Author information: Elmer W. Koneman, MD, received his graduate degree from the University of Colorado School of Medicine in Denver, Colorado, and until his recent passing, was a Professor Emeritus at their Department of Pathology. He was the author of numerous journal articles and books, including Color Atlas and Textbook of Diagnostic Microbiology, 7th Edition, 2017. 
Author information: Margaret Reinhart MS, MT(ASCP) is the MLS Program Director and Senior lecturer in Biological Sciences at the University of the Sciences in Philadelphia PA where she teaches hematology, clinical immunology, parasitology, and other related courses. She is also an adjunct instructor in Hematology at Pennsylvania Hospital, Philadelphia PA. She holds a Masters Degree in Biology and in Health Care Administration.
Reviewer information: Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration Healthcare System, and has prior experience as an Administrative Laboratory Director.
 
Content information: This course describes and identifies medically important yeast and dimorphic pathogens. Many photomicrographs are used to assist with the recognition of organisms in culture and microscopically.

Keywords

These are the most common topics and keywords covered in Mycology: Yeasts and Dimorphic Pathogens:

histoplasmosis microconidia inoculate fungemia immunocompromised assimilation macroconidia coccidioidomycosis methods ulcerating caves endemic carcasses arthroconidium incubation immitis diagnosis fungal molds cryptococci brown-black inhalation nucleic bird membranes bats yellow-white tinea multilateral coccidioides clusters blood germ photomicrograph necks mount hockey protein saccharomyces kefyr 35#176-37#176c lollipops orange-pink organism intravenous arthroconidia dimorphic prowl diagnostic respiratory mariners echinulate cob-web cerevisiae materials lollipop versicolor prickly blastomyces blastospores morphologies daisy-head histoplasma mycelium septation antifungal blastoconidia dubliniensis torulopsis river culture wrinkled ascospore laboratory glucose caffeic parapsilosis conidiophore aerosolization sputum shunt daisy pigmentation burrows matchstick pseudohyphae chlamydoconidia malassezia capsulatum peritoneal dermatitidis conidium rubra rats polypeptide cottony sabourauds blastomycosis gray-green thick-walled olive saprophytic cryptococcus spherules drop-down fungi vaginal hyphae furfur serum capsules filamentous candida schenckii endospores bovine broad-based flower chlamydospores biopsy intraventricular morphologic morphology lactophenol fungus glabrous urine alveolar mucoid decaying heart glabrata phenoloxidase neoformans alternately agar yeasts identification stick budding contracted albicans trehalose reproduces gray-white desert colony hyphal dextrose cutaneous subculture infections disease catheter pathogen cornmeal petals logs inoculated hemoglobin kentucky sporothrix buds brasiliensis brain successively trichosporon infection body mississippi conidia ribosome mucous geotrichum yeast-like pseudotropicalis rodents dialysis eukaryotes laurentii cycloheximide valleys clinical downy paracoccidioides ascospores fetal rectangular red-brown specimens polysaccharide differential appears attachments saprophytes cells non-pigmented guano soil organisms rhodotorula
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 154 CE courses, most popular
$95Add to cart
Pick Your Courses
Up to 8 CE hours
$50Add to cart
Individual course$20Add to cart
Need multiple seats for your university or lab? Get a quote
Candida parapsilosis, cornmeal agar mount.  Characteristic is the production of several spider-like colonies, composed of delicate pseudohyphae, produced along the margins of the streak.


dimorphic fungi yeast forms microscopic


question Blastomyces


lollipop conidia


yeast01


YEAST02


Yeast03


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