Course Outline
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- Introduction to the Intestinal Parasitic Protozoa
- Overview of Diagnostic Techniques
- Types of Diagnostic Techniques
- Collection and Processing for Performing the Microscopic Exam
- Collection and Processing for Performing the Microscopic Exam: Fixatives
- Concentration Techniques
- How to Perform the Microscopic Exam
- Entero-Test (Duodenal Capsule)
- Molecular Methods
- Antigen and Antibody Detection
- Suggested Protocols for Parasite Recovery
- True or False: A direct wet prep for examining stool specimens for ova and parasites is a preferred method for parasite detection and identification.
- All of the following are stains that can be used on permanent smears for protozoa except:
- Life Cycles, Epidemiology, Diseases, and Diagnosis of the Amoebae
- Pathogenic versus Commensal Amoebae
- Entamoeba histolytica Life Cycle
- Entamoeba histolytica Epidemiology and Disease
- Differentiating E. histolytica Trophozoites from the Commensal Amoeba Trophs
- Differentiating E. histolytica Cysts from the Commensal Amoeba Cysts
- Other Diagnostic Techniques for Entamoeba histolytica
- Match the amoeba species with whether it is pathogenic or commensal.
- True or False: People become infected with Entamoeba histolytica by consuming food contaminated (usually of human fecal origin) with trophozoites of t...
- An MLS is puzzled when she finds cysts resembling E. histolytica or E. hartmanni. A way of distinguishing Entamoeba histolytica cysts from cysts of En...
- Common methods of detecting pathogenic gastrointestinal infection with Entamoeba histolytica include all of the following except:
- Life Cycles, Epidemiology, Diseases, and Diagnosis of the Flagellates
- Life Cycle, Epidemiology, Disease, and Diagnosis of the Ciliate Balantidium coli
- Other Intestinal Protists
- Blastocystis spp: Epidemiology, Disease, and Life Cycle
- Blastocystis Diagnosis
- Intestinal Sporozoa: Cryptosporidium spp., Cyclospora cayetanensis, Cystoisospora belli, and Sarcocystis spp.
- Cryptosporidium spp.
- Cyclospora cayetanensis
- Cystoisospora belli (formerly Isospora belli)
- Sarcocystis spp.
- Microsporidia
- Summary of the Morphologic Features of Blastocystis, Sporozoa, and Microsporidia
- The sporozoa are characterized by all of the following except:
- A laboratorian performs an acid-fast stain on a fecal smear and finds several round, acid-fast structures measuring 8–10 μm. The most likel...
- True or False: The organisms belonging to the Microsporidia group have an obligate extracellular phase of development.
- References
Additional Information
Level of Instruction: Intermediate to Advanced
Intended Audience: Medical laboratory scientists, medical laboratory technicians, laboratory supervisors, and laboratory managers. This course is also appropriate for MLS and MLT students and pathology residents.
Author Information: Margaret Reinhart, MS, MLS(ASCP), is a Senior Lecturer emerita in Biological Sciences at the University of the Sciences in Philadelphia, PA, where she taught hematology, clinical immunology, parasitology, and other related courses, as well as directed the MLS program for over 30 years. She is currently an adjunct instructor in Hematology at Pennsylvania Hospital, Philadelphia, PA. She holds a Master's Degree in Biology and Health Care Administration.
The author has no
conflict of interest to disclose.
Reviewer Information:
Diane M. Valentin, MS, MLS(ASCP)MB, is a graduate of the University of the Sciences in Philadelphia who has worked as a medical laboratory professional since graduation. She also earned a Master of Science degree in biomedical informatics, focusing on bioinformatics, from Rutgers University and a certificate in molecular laboratory diagnostics from Michigan State University. She holds two certifications from the American Society of Clinical Pathology as a Medical Technologist and Technologist in Molecular Biology.
Laurie Bjerklie, MA, MLS(ASCP)CM, is
a Lead Education Developer. She earned a B.S. in Medical Laboratory Science
from the University of North Dakota and an M.A. in Curriculum and Instruction
from Saint Xavier University. She has over 15 years of experience in higher
education and has held program director and faculty positions in both MLT and
MLS programs.
