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Free-Living Amoeba as Agents of Infection (Online CE Course)

(based on 625 customer ratings)

Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB); Tamika E. Gosha, BS, MLT(ASCP)
Reviewers: Judi Bennett MT, BSM and Alexandru Casapu, MBA, MLS(ASCP)CM

Most protozoa are free-living and have little or no impact on human health. They are found throughout the environment, particularly in soil and water. However, there are four free-living amoeba that have been associated with serious human infections. This course seeks to identify these amoeba and discuss their life cycles, methods of infection, and the symptoms that occur, as well as determine preventive measures that may be available.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 4/30/2022
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Microbiology/Mycology/Parasitology): 1 hour(s)
Approved through 9/1/2022


  • Identify the primary free-living amoeba that infect humans and contrast their life cycles.
  • Recognize the method of infection and symptoms that occur for each of the free-living amoeba reviewed.
  • Determine preventive measures for each of the free-living amoeba.

Customer Ratings

(based on 625 customer ratings)

Course Outline

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Additional Information

Level of instruction: Basic
Intended Audience: Medical laboratory scientists, medical technologists, and technicians. This course is also appropriate for medical laboratory science students and pathology residents. 
Author information: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB) is Laboratory Director & Director of Laboratory Operations, Quest Diagnostics-Valdosta RRL. Dr. Pendergraph is the laboratory director for Quest Diagnostics in Valdosta, GA and Miller County Hospital in Colquitt, GA. Dr. Pendergraph received his MSPH from the University of Kentucky in Lexington, his PhD in medical parasitology and mycology from the University of  North Carolina in Chapel Hill and his law degree with a concentration in health care law from Concord Law School, Kaplan University. He also did a Fellowship in Tropical Medicine at Louisiana State University School of Medicine. He is the author of a textbook in phlebotomy, a number of scientific articles, plus internet training programs.
Co-auther information: Ms. Tamika Gosha is a medical laboratory technician at Quest Diagnostics, Inc./Solstas Lab Partners Valdosta laboratory. She is presently working on her master’s degree at Kaplan University.
Reviewer information:  Judi Bennett MT, BSM is currently a Program Director for MediaLab, Inc. in Lawrenceville, GA. She has over 30 years of medical laboratory experience in an acute care hospital setting as a laboratory manager, senior clinical applications specialist, point-of-care coordinator, microbiology supervisor, and generalist technologist. 
Reviewer Information: Alexandru Casapu, MBA, MLS(ASCP)CM, PBTCM has over 20 years of experience as an MLS, section supervisor, and laboratory manager. A former Director of Clinical Laboratory Technology Program at Georgia Piedmont Technical College, he is currently a Program Director at MediaLab, Inc. 

<em>N. fowleri</em> culture - CDC
<em>B. mandrillaris</em> cyst
amoeba nucleolus
Trophozoites in brain biopsy
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<em>N. fowleri</em> culture - CDC

<em>B. mandrillaris</em> cyst

amoeba nucleolus

Trophozoites in brain biopsy