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Vitamin D (Online CE Course)

(based on 405 customer ratings)

Author: Lynne Brodeur, MA, MLS(ASCP)CM
Reviewers: Kevin F. Foley, PhD, DABCC, MT, SC; Krista Moore, MLS(ASCP)CM

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Course number 578-008-19, approved through 2/28/2021
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1 hour(s)
Course number 20-705006, approved through 9/1/2020


  • Differentiate between the various forms of vitamin D.
  • Explain the mechanisms of metabolism of vitamin D.
  • Discuss vitamin D deficiency, screening, and risk factors.
  • List the various methodologies for testing vitamin D.
  • List the analytical variables that may affect vitamin D testing.

Customer Ratings

(based on 405 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • The Different Forms of Vitamin D
      • Role of Vitamin D
      • Receptors
      • Vitamin D Forms
      • Vitamin D2 Form
      • Vitamin D3 Form
      • Vitamin D is considered biologically inactive until it undergoes two enzymatic hydroxylation reactions. Where do these reactions take place?
      • The active form of vitamin D is called:
  • Vitamin D Sources and Metabolism
  • Causes of Vitamin D Deficiency
  • Diseases Associated with Decreased Vitamin D
      • Children (Growth and Development)
      • Hyperparathyroidism
      • Osteoporosis and Osteopenia
      • Immune System Impairment
      • Rickets
      • Vitamin D is NOT responsible for building strong bones and teeth in children.
      • Dorothy is a 70-year-old female with the following test results:Vitamin D = 9 ng/mL (20 - 50 ng/mL)Calcium = 7.0 mg/dL (8.5 - 10 mg/dL)Based upon thes...
  • Vitamin D Testing Methods and Methodologies
      • Historical Measurements
      • The Reference Method
      • HPLC
      • Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
      • Immunoassays
      • Sample Requirements
      • Which types of samples would be most appropriate for vitamin D testing?
      • What are some of the advantages of using liquid chromatography tandem mass spectrometry (LC - MS/MS) versus traditional immunoassay techniques?
  • Interferences
      • Medications
      • Antibody and Other Interferences
      • Heterophile antibodies may cause erroneous results in assays that utilize antibodies as their methodology.
      • A patient from the dialysis unit has a vitamin D ordered along with BUN and creatinine. The laboratory request form that accompanies this sample state...
  • References
      • References

Additional Information

Level of Instruction: Intermediate

Intended Audience: Medical laboratory scientists, medical laboratory technicians, pathology residents, MLS students, and other health care personnel who have an interest in this subject matter.

Author Information: Lynne Brodeur, MA, MLS(ASCP)<sup>CM</sup> holds a master of arts degree in teaching. She is currently a full time lecturer at the University of Massachusetts Dartmouth and works per diem in Clinical Chemistry at St. Luke's Hospital in New Bedford, MA.

Reviewer Information:  Kevin F. Foley, PhD, DABCC, MT, SC is the director of clinical pathology for the Kaiser Permanente Northwest region. He also teaches clinical chemistry at Oregon Health Sciences University. Dr. Foley earned his PhD in clinical pharmacology and toxicology at East Carolina School of Medicine in North Carolina.

Krista M. Moore, MLS(ASCP)<sup>CM</sup> is a Technical Specialist for Chemistry, Immunology, and Toxicology at Kaiser Permanente, Portland, Oregon. She holds BS degrees in Clinical Laboratory Science from Oregon Health Science University/Oregon Institute of Technology and in Chemistry from State University of NY at Geneseo. 

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vitamin D metabolism
CLIA Vitamin D assay example
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vitamin D metabolism



CLIA Vitamin D assay example