Chemical Screening of Urine by Reagent Strip (Online CE Course)

(based on 416 customer ratings)

Author: Leah Westover, MPH, MLS(ASCP)
Reviewer: Joshua J. Cannon, MS, MLS(ASCP)CMSHCM

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This course discusses the chemical tests that are an integral part of a urinalysis. Proper specimen handling and technique are discussed for key tests, including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, and leukocyte esterase.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Approved through 1/31/2026
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Clinical Chemistry/UA/Toxicology): 1.5 hour(s)
Approved through 1/31/2026

Objectives

  • Define the optimal storage time and temperature for urinalysis samples.
  • Review the proper storage of and procedure for the use of urinalysis reagent strips.
  • Describe the chemical reactions, quality control measures, and interpretation of results for urinalysis reagent strip analytes including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, leukocyte esterase.
  • Explain the proper techniques used during urinalysis reagent strip testing; ensuring accuracy.
  • Identify conditions and/or substances that may interfere with urinalysis reagent strip testing.
  • Identify the additional testing protocols used to confirm urinalysis reagent strip testing results.
  • Summarize the analytical principles of measurement in urinalysis reagent strip instruments.

Customer Ratings

(based on 416 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Introduction to Urine Chemical Reagent Strips
      • Chemical Urinalysis Reagent Strips
      • Urine Specimen Collection
      • Urine Specimen Transport
      • Reagent Strip Procedure: Manual or Semi-Automated Methods
      • Handling and Storage of Urine Reagent Strips
      • Which of the following statements is true regarding the storage and handling of urine reagent strips?
      • All the following statements about the urine specimen are true except:
      • Which of the following statements is true for the reagent strip procedure?
      • True or False: Quality control procedures should be performed with each new lot of chemical reagent strips and as often as required by the laboratory'...
  • Urine pH Analysis
      • pH Analysis: The Urine Specimen
      • pH Reaction
      • Urine pH: Acidic and Alkaline
      • A urine specimen was collected at 6:00 AM and remained at room temperature until it was received in the laboratory at 3:30 PM. How may the pH of the s...
      • Match the following factors with the expected urine pH type.
  • Urine Specific Gravity Analysis
  • Urine Protein Analysis
      • Protein Error of Indicators
      • Confirmatory Testing for Urine Protein
      • Clinical Significance of Urine Protein
      • Clinical Significance of Urine Protein, continued
      • Follow-up Testing for Positive Urine Protein
      • The presence of increased levels of protein in the urine may be an early indicator of:
      • Which of the following conditions is associated with benign proteinuria?
  • Urine Glucose Analysis
  • Urine Ketone Analysis
      • Ketones Overview
      • Ketone Reaction
      • False-Positive and False-Negative Ketone Test Results
      • Clinical Significance of Urine Ketones
      • The ketone component that is measured by the nitroprusside reaction is:
      • Which of the following can cause a false positive result for ketones?
      • Significantly increased levels of ketones are detected in urine in all of the following conditions except:
  • Urine Blood Analysis
      • Introduction to Hematuria
      • Blood Reaction
      • False-Positive and False-Negative Blood Results
      • Clinical Significance of Blood in Urine
      • A urine sample appears cloudy and pink. The microscopic examination reveals the presence of intact red blood cells. What is the term that is used to d...
      • Which of the following substances can cause a false positive result for blood on the urine reagent strip?
  • Urine Nitrite Analysis
  • Urine Leukocyte Esterase
  • Urine Bilirubin Analysis
      • Overview of Bilirubin
      • Bilirubin Reaction
      • False-Positive and False-Negative Urine Bilirubin Results
      • Ruling out False-Positive Urine Bilirubin Test Results
      • Clinical Significance of Urine Bilirubin
      • Which of the following may cause a false-positive bilirubin result on a urine chemical reagent strip?
      • When a patient has a bile duct obstruction, the bilirubin test portion of the reagent strip is:
      • Which form of bilirubin is indicated by a positive urine reagent strip test?
  • Urine Urobilinogen Analysis
      • Introduction to Urobilinogen
      • Urobilinogen Reaction
      • False-Positive and False-Negative Urobilinogen Results
      • Clinical Significance of Urobilinogen in Urine
      • True or False: Urobilinogen production is low or absent in biliary obstruction, which will be detected on the urine dipstick.
  • Automation of Chemical Reagent Strip Analysis
      • Semi-Automated Instruments
      • Automated Systems
      • Quality Control
      • True or False: Urine chemical reagent strip readers can utilize any manufacturer's dipsticks.
      • When an automated or semiautomated method is used to read urine chemical reagent strips, quality control testing must be performed:
  • Summary Tables
      • Test Principles, Reactions, and Interfering Substances
  • References

Additional Information

Level of Instruction: Basic
Intended Audience: Medical laboratory science and medical laboratory technician students and professionals, clinical laboratory personnel, and other medical personnel who perform urine dipstick testing at the point of care.
Author Information: Leah Westover, MPH, MLS(ASCP) is the Director of Sales and Marketing for MediaLab, Inc. Previously, she was the Director of Education and Outreach as well as the Medical Laboratory Science Program Director at St. Christopher’s Hospital for Children in Philadelphia, Pennsylvania. Leah holds a Masters in Public Health from Thomas Jefferson University.
Reviewer Information: Joshua J. Cannon, MS, MLS(ASCP)CMSHCM received his Bachelor of Science and Master of Science in Medical Laboratory Science from Thomas Jefferson University in Philadelphia, PA. He holds Medical Laboratory Scientist and Specialist in Hematology certifications through the ASCP Board of Certification. He was a professor at Thomas Jefferson University for seven years before transitioning into his current role as Education Developer at MediaLab. His areas of expertise and professional passions include clinical hematology and interprofessional education.
 

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 178 CE courses, most popular
$109Add to cart
Pick Your Courses
Up to 8 CE hours
$55Add to cart
Individual course$25Add to cart
Individual course$25Add to cart
Need multiple seats for your university or lab? Get a quote
Chemical Screening of Urine by Reagent Strip


2 urine microscopic tubes in blue rack sml


orange lid sterile urine cup with urine 2 sml


urine strip almost dipping into urine cup sml


urine strips with controls sml


timer for urinalysis sml


Correct storage


urine strip vertical in front of strip bottle and urine sml