Course Outline
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- Clinical Indications
- Bone Marrow Examination: Clinical Indications
- Bone Marrow Procurement
- Bone Marrow Procurement
- Bone Marrow Procurement: Supplies
- Bone Marrow Procurement: Aspirate
- Bone Marrow Procurement: Core Biopsy
- Aspirate: Optional Immediate Bedside Smear Preparation
- Core Biopsy: Optional Immediate Bedside Imprint Preparation
- In adult patient populations, what is the preferred site for the collection of bone marrow aspirates and biopsies?
- True or False: The core biopsy should be red, with grossly identifiable bone particles.
- Bone Marrow Core Imprints
- Bone Marrow Core Imprints (Touch Preparation)
- Bone Marrow Aspirate Preparation: Anticoagulated Sample
- Bone Marrow Aspiration: Laboratory Technical Preparation
- Myeloid-Erythroid Cells/Concentrate/Buffy Coat Smears
- To prepare the myeloid-erythroid (M-E)/concentrate/buffy coat smears, a clean pipette should be used to make the concentrate. After pipetting off the ...
- Bone Marrow Clot Preparation
- Bone Marrow Clot Preparation
- True or False: The clot section includes the remaining particles from the aspirate and red blood cells. Three levels, usually sectioned at 3 microns t...
- Bone Marrow Core Biopsy
- Bone Marrow Core Biopsy
- True or False: Bone marrow core biopsy specimens do not allow for the best evaluation of bone marrow cellularity, cellular distribution, cellular rela...
- Iron Stain on Spun Bone Marrow Aspirate Fat-Perivascular Layer
- Iron Stain on Spun Bone Marrow Aspirate Fat-Perivascular Layer
- True or False: In order not to disrupt fat cells, alcohol fixation of the fat-perivascular (F-PV) slide used for iron store evaluation with the Prussi...
- Iron Stain on Bone Marrow Concentrate (Myeloid-Erythroid Layer)
- Technical Preparation of Iron Stain on Bone Marrow Concentrate (Myeloid-Erythroid Layer)
- Immunohistochemical Stains
- Metastatic Carcinoma Immunohistochemical Stains
- CD138 Immunohistochemical Stain
- CD34 Immunohistochemical Stain
- CD117 or Mast Cell Tryptase Immunohistochemical Stains
- Special Stains
- Reticulin Stain
- Prussian Blue Stain
- Bacterial and Fungal Stains
- Amyloid Stain
- Which stain is commonly utilized to detect iron in bone marrow specimens?
- True or False: Amyloid deposits are most commonly identified on core biopsy specimens, but may also be seen on aspirate smears.
- Histochemical Stains
- Enzyme Stains
- True or False: The application of enzyme/histochemical stains by the pathologist will not change the classification of the disease in a bone marrow bi...
- Esoteric Testing
- References
Additional Information
Level of Instruction: Basic to intermediate
Intended Audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and medical laboratory science students, pathology residents, and practicing pathologists.
Author Information: Brooke Eguia, MS, BS, HT(ASCP), HTL(ASCP), is the Pathology Technical Supervisor at Fairview Southdale Hospital in Edina, Minnesota. She graduated from St. Cloud State University with a Bachelor of Science degree in general biology and from Capella University with a Master of Science in Human Service with a specialization in Health Care Administration. During her time as an undergraduate, Brooke's interests and studies focused on histologic techniques and gross anatomic dissection. Acting as a primary health career mentor to high school students, Brooke satisfied her desire for training and teaching histotechnicians and also worked as adjunct faculty at Rasmussen College, as a Medical Assistant laboratory techniques instructor. Most recently, she has focused on proctoring histology students in clinical/classroom progress and exam preparation.
The author has no
conflict of interest to disclose.
Reviewer Information: Kristen Meyer, BS, HTL(ASCP), QIHC(ASCP), is the Manager of Anatomic Pathology Services for Kaiser Permanente in Portland, OR. She graduated from Tulane University with a Bachelor of Science degree in Biology. Upon graduation, she began working in a hospital as a clinical lab assistant, and from there became interested in Histotechnology after observing gross dissections of specimens in the Pathology department. Since then, she has worked in the Histopathology field for over 20 years in various roles, including Histotechnologist, Technical Specialist, Supervisor, and Manager.
Course Description: This course provides information about the clinical indications for bone marrow examination and detailed instruction for preparing bone marrow smears used for histological testing.
