Common Cytoplasmic Staining Patterns, continued

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The page below is a sample from the LabCE course Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns (online CE course)
Common Cytoplasmic Staining Patterns, continued

Anti-Golgi pattern (AC-22)
Coarse granular staining around one side of the nucleus is characteristic of an anti-Golgi antibodies pattern. This pattern is reported as "ANA negative, suspect anti-Golgi antibodies present." No follow-up testing is necessary. Anti-Golgi antibodies are seen in various diseases. An example of such a fluorescent staining pattern is on the right.
Anti-mitotic spindle pattern
Anti-mitotic spindle antibodies refer to several different patterns, which in a broad sense, include CENP-F, Centromere, and Midbody. However, in the case of cytoplasmic patterns, it only applies to the staining of the spindle fibers. This pattern may appear in various autoimmune disorders. It is reported as "Suspect mitotic spindle."
Anti-NuMA pattern (AC-26)
This pattern produces a speckled ANA along with staining of the spindle fibers. It is reported as "ANA positive, speckled, suspect mitotic spindle antibodies present." Follow-up testing to rule out possible antibodies to the ENAs is suggested. Titering is necessary.
Anti-HsEg5 pattern (AC-25)
Antibodies to HsEg5 are ANA negative and only stain the spindle fibers. This pattern is reported as "ANA negative, suspect mitotic spindle antibodies present." No follow-up testing is necessary and titering is optional.