ANA Patterns

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The page below is a sample from the LabCE course Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns (online CE course)
ANA Patterns

The following pages will contain photographs of various ANA patterns. These photomicrographs were taken of actual patient samples using indirect immunofluorescence (IFA) and the Colorzyme® methods.
As described previously, the Colorzyme® method uses a horseradish peroxidase (HRP) conjugate, and the results are read using brightfield microscopy.
In an effort to standardize the interpretation of ANA patterns2, the Autoantibody Standardization Committee, a subcommittee of the International Union of Immunological Societies (IUIS) Quality Assessment and Standardization Committee in conjunction with the Centers for Disease Control and Prevention (CDC), formed the International Consensus on ANA Patterns (ICAP). This group has issued a standardized set of ANA photographs for nuclear, cytoplasmic, and mitotic HEp-2 cell patterns designated AC-1 through AC-28.
2. Chan EK, Damoiseaux J, Carballo OG, Conrad K, de Melo Cruvinel W, Francescantonio PL, Fritzler MJ, Garcia-De La Torre I, Herold M, Mimori T, Satoh M, von Mühlen CA, Andrade LE. Report of the first international consensus on standardized nomenclature of antinuclear antibody HEp-2 cell patterns 2014-2015. Front Immunol. Aug. 20, 2015; 6:412,