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The page below is a sample from the LabCE course Semen Analysis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Summary: Reference Values

The following are lower reference limits for a normal semen analysis. It should be noted that these are recommendations only, as stated in World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th ed. The limits may differ from those used in your laboratory, if laboratory studies or different reference materials were used to establish the values.

  • Liquefaction: ≤30 minutes (no greater than 1 hour)
  • Volume: >1.5 mL
  • pH: ≥7.2
  • Sperm concentration: ≥15 x 106 / mL
  • Motility: Progressive motility 32%. The lower reference limit for total motility (progressive + non-progressive) 40%.
  • WHO 5th edition: ≥4% normal forms