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The page below is a sample from the LabCE course HIV: Structure, Replication, and Detection. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Anti-HIV Immunoassays

Immunoassays that are currently available detect antibodies to both HIV-1 and HIV-2, using antigens specific for both viruses. Some tests also include group O-specific antigens to increase sensitivity to group O infections. Test results that are at or above the cutoff value are considered initially reactive for antibodies to HIV-1 or HIV-2.
Initial (screening) tests are highly sensitive and will produce few false-negative results, if seroconversion has occurred. However, immunoassays may lack specificity.
All current HIV screening assays used in the U.S. will detect both HIV-1 and HIV-2.