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The page below is a sample from the LabCE course HIV: Structure, Replication, and Detection. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Western Blot

Western blot had been the gold standard for HIV confirmation for many years; however, it is no longer part of the Centers for Disease Control and Prevention (CDC) recommended algorithm for confirmation of reactive initial immunoassay results. Western blot can produce false-negative results in late infection (as antibody titers diminish) or indeterminate results early in the course of HIV infection. An indeterminate result is frustrating to a patient and clinician as there is much anxiety around the confirmation of an HIV diagnosis.