This is an example of a fatty/brain processing protocol with a closed-system processor, using standard processing reagents and also using pressure/vacuum. The tissue has been mostly fixed prior to processing. Notice how much more time fatty tissue requires in each reagent. Typically, fatty tissue requires approximately 4 or 5 hours MORE processing time than routine surgicals. Fatty tissue that is processed on a routine processing protocol may need to be reprocessed if the tissue is found to be mushy or greasy.
10% neutral buffered formalin (NBF) | 90 |
10% NBF | 90 |
70% alcohol | 60 |
95% alcohol | 90 |
95% alcohol | 90 |
100% alcohol | 100 |
100% alcohol | 100 |
100% alcohol | 120 |
Xylene | 100 |
Xylene | 120 |
Paraffin | 90 |
Paraffin | 90 |
Paraffin | 90 |
Paraffin | 90 |