The page below is a sample from the LabCE course Tissue Processing in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Fatty Tissue Processing

This is an example of a fatty/brain processing protocol with a closed-system processor, using standard processing reagents and also using pressure/vacuum. The tissue has been mostly fixed prior to processing. Notice how much more time fatty tissue requires in each reagent. Typically, fatty tissue requires approximately 4 or 5 hours MORE processing time than routine surgicals. Fatty tissue that is processed on a routine processing protocol may need to be reprocessed if the tissue is found to be mushy or greasy.
ReagentTime (minutes)
10% neutral buffered formalin (NBF)90
10% NBF90
70% alcohol60
95% alcohol90
95% alcohol90
100% alcohol100
100% alcohol100
100% alcohol 120
Xylene100
Xylene120
Paraffin90
Paraffin90
Paraffin90
Paraffin90