Segmented neutrophils are also referred to as segs, polymorphonuclear leukocytes, polys, and PMNs. Segmented neutrophils are the most mature neutrophilic granulocytes present in circulating blood. The diameter of a segmented neutrophil is approximately 9-15 microns and the N:C ratio is approximately 1:3.
The cytoplasm of a segmented neutrophil resembles the cytoplasm of a band. It stains faintly pink and contains numerous fine specific granules, which stain pinkish-lilac with Wright or Giemsa stain.
A segmented neutrophil has the following characteristics:
- The nucleus is a deep purple color, and the chromatin has a coarse, clumped texture.
- The nucleus has from 2-5 lobes, with an average of 3.
- The lobes are connected to each other by a fine filament or strand of nuclear membrane.
- A filament is a thread-like strip, which is so narrow that there is no visible nuclear material between the two sides.