Ketone Reaction

This version of the course is no longer available.
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course . Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about (online CE course)
Ketone Reaction

The test for ketone bodies is based on a nitroprusside reaction. Acetoacetic acid reacts with sodium nitroferricyanide and glycine in an alkaline medium to produce a violet-to-purple colored complex. The ketone pad on the reagent strip can detect as little as 5 mg/dL acetoacetic acid in urine. It does not react with acetone or B-hydroxybutyric acid.