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Ketone Reaction

Testing for ketone bodies is based on a nitroprusside reaction. Acetoacetic acid reacts with sodium nitroferricyanide and glycine in an alkaline medium to produce a violet-to-purple colored complex. The ketone pad on the reagent strip can detect as little as 5 mg/dL acetoacetic acid in urine. It does not react with acetone or B-hydroxybutyric acid.