Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Antinuclear Antibody Testing: Methods and Pattern Interpretation. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Antinuclear Antibody Testing: Methods and Pattern Interpretation (online CE course) »
How to Subscribe
Individual course$20 Add to cart

Homogeneous and Speckled

This is an example of a mix of homogeneous and speckled ANA patterns.

In this sample notice the Speckled ANA is the dominant pattern in the interphase cells (a) and specklingis also seen outside of the chromosomal area of the mitotics (b).

Also notice the smooth staining of the chromosomal area of the metaphase mitotic cells (c). This represents the presence of a homogeneous ANA pattern.

In cases of mixed homogeneous and speckled ANAs follow-up testing for anti-dsDNA and anti-ENA antibodies is necessary.

When mixed patterns are titered, the endpoint for each pattern is reported.

This pattern is reported as ANA positive, Homogeneous and Speckled; titering is necessary.

Homogeneous and Speckled:

Interphase cells

  • Often more speckled than homogeneous
  • Nucleoli may or may not stain

Metaphase mitotic cells

  • Smooth staining of the chromosomal region
  • Region outside of the chromosome area will stain with variable intensity