The formula for calculating the sperm count, when 5 small squares within the large center square are counted is:
Number of sperm counted x dilution factor/volume x 1000 = sperm/ml.
Example: 50 sperm are counted in the five small squares. The dilution is 1:20.
Sperm/ml = 50 x 20/0.02 mm3 x 1000 mm3/ml = 50,000,000 sperm/ml.
If the dilution is 1:20, and the usual 5 small squares are counted, then the formula can be simplified as follow:
Sperm/ml = Sperm counted x 1,000,000.
