Calculating Percent Volume/Volume (% v/v)

The page below is a sample from the LabCE course Mathematic Calculations and Applications for the Histology Laboratory. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Mathematic Calculations and Applications for the Histology Laboratory (online CE course)
Calculating Percent Volume/Volume (% v/v)

A percent v/v solution is calculated by the following formula using the milliliter as the base measure of volume (v):
% v/v = mL of solute/100 mL of solution
Example:
What is the % v/v of a solution that has 5.0 mL of hydrochloric acid (HCl) diluted to 100 mL with deionized water?
X% = 5.0 mL HCl / 100 mL of solution
X / 100 = 5.0 / 100
Cross multiplying, 100X = 500
X = 5.0% v/v