Mobility or Rate of Migration

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The page below is a sample from the LabCE course Electrophoresis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Mobility or Rate of Migration

The mobility or rate of migration of ions in electrophoresis is dependent upon the following factors:
  • Net charge of the molecules
  • Size and shape of the molecules
  • Support medium properties
  • Strength of the electrical field
  • Ionic strength of the buffer
  • Temperature