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The page below is a sample from the LabCE course Tissue Decalcification for Paraffin Processing. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Weak Organic Acids

Formic acid formulations and properties:
  • Available as 5%-10% aqueous, 25% formic with sodium citrate, 4% formic with 4% HCl
  • Most common decalcifier
  • Slower acting
  • Good staining results
  • Economical
  • Staining acceptable after prolonged exposure
  • Formic acid and formaldehyde is a common fixative/ decal combination