Other Tests: Enzyme-Linked Immunosorbent Assay (ELISA)

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The page below is a sample from the LabCE course Laboratory Evaluation of the Lupus Anticoagulant found in Antiphospholipid Syndrome (APS). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Laboratory Evaluation of the Lupus Anticoagulant found in Antiphospholipid Syndrome (APS) (online CE course)
Other Tests: Enzyme-Linked Immunosorbent Assay (ELISA)

Not all antiphospholipid antibodies can be detected by clotting tests and require immunochemical ELISA tests. Testing is performed for anti-β2 glycoprotein I (anti2GPI) and anticardiolipin (aCL).
The ELISA method uses a plate coated with either β2GPI or phospholipid cardiolipin to which the patient serum is added and then sandwiched with a secondary labeled antibody that allows quantitation of the bound antibodies.
If a positive test is obtained, it is recommended that the assay be repeated after 12 weeks to eliminate any false positive results.