Diagnosis and Identification of Fast-Growing Molds

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The page below is a sample from the LabCE course Mycology: Hyaline and Dematiaceous Fungi. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Diagnosis and Identification of Fast-Growing Molds

The following chart describes fast-growing molds which are less often pathogenic.
OrganismDisease and Site Identifying FeaturesOther Comments
Alternaria spp. Phaeohyphomycosis, sinus/nasal infection Hyphae dematiaceous and septate. Golden brown hyphae have conidiophores with a chain of conidia resembling a drumstick. Produce dictyospores
Usually not pathogenic
Aureobasidium pullulansSkin, nails. Occasional phaehyphomycosis
Black yeast-like colonies. Conidia are hyaline and smooth-walled, single-celled ellipsoidal and variable shape and size (8-12 x 4-6 μm). Often has an indistinct hilum
Note: Aureobasidium pullulans and Hortaea wernickii characteristically produce black yeasts throughout their maturation process. The young colonies of Exophiala jeanselmei may appear as a black yeast, being microscopically identical to Hortaea wernickii.
Bipolaris spp. Phaeohyphomycosis, chronic sinusitis
Hyphae dematiaceous and septate. Conidiophores are bent where conidia attached. Multicelled macroconidia are oblong to fusoid and smooth walled and are pseudoseptate (diptosepta). Can produce polar germ tubes.
Note: The macroconidia of Drechslera species are muriform - showing both longitudinal and transverse septation. They appear similar in many ways to Bipolaris, except that they appear longer and thinner. (Drechslera species also produce germ tubes upon incubation; however, they are single and emerge only from the hilar cell at a right angle.)
Usually not pathogenic
Curvularia spp. Phaeohyphomycosis, sinusitis Hyphae dematiaceous and septate. Conidiophores are bent where conidia are attached. Macroconidia are brown, curved (boomerang- shaped) with a central enlarged cell; end cells are lighter and have distinct transverse septa
Epicoccum spp. None; generally a clinical contaminant When sporulating, numerous black sporodochia (aggregates of cushion-like conidiophores) are visible. Macroconidia are divided by longitudinal and transverse septa (dictyospores).
Exserohilum spp. Phaeohyphomycosis Hyphae dematiaceous and septate. Conidiophores are bent where conidia attached. Multi-celled macroconidia are long, pencil shaped and have a distinctive hilum (hilar cell extension)and have transverse septae.
Nigrospora spp. Rarely pathogenic; usually a plant pathogen Colony starts out white and wooly, but then darkens with maturity, turning black or brown. Single, globose, black conidia.
Scedosporium boydii (previously known as Pseudallescheria boydii)Mycetoma, Phaeohyphomycosis Has both sexual and asexual reproduction
Conidia are golden brown and single-celled, and borne from tips of conidiophores
Spores are in cleistothcia
See more info under the hyaline mold section.
Scopulariopsis brumptii
(other notable species include: S. brevicaulis, and S. gracilis.)
Rare cause of disease Dematiaceous variation of the hyaline mold, Scopularopsis spp. Produces chains of large, lemon-shaped conidia with darkly staining bases, called annelloconidia:
(S.brumptii)
Stemphylium spp. Rare cause of disease Dark brown, oblong, multicelled muriform conidia supported by a straight conidiophore bale of cotton on a stick
(images courtesy of U. of Adelaide)
Ulocladium spp. Keratitis Numerous, usually solitary, multicelled divided conidia (dictyoconidia or dictyospores) are formed through a pore (poroconidia) by a sympodially elongating geniculate (bent) conidiophore(which differentiates it from Stemphylium)
(second image courtesy of U. of Adelaide)