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The page below is a sample from the LabCE course HIV: Structure, Replication, and Detection. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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The Centers for Disease Control and Prevention (CDC) Recommended Algorithm for HIV Testing

The CDC recommends that everyone between the ages of 13 and 64 get tested at least once as part of routine health care. For those with specific risk factors, the recommendation is testing once a year.*
In 2014, the CDC published a new HIV testing algorithm. The 2018 update of the algorithm is shown below and can be found on the CDC website at:
HIV/AIDS laboratory tests. Centers for Disease Control and Prevention website. Available at: https://www.cdc.gov/hiv/testing/laboratorytests.html. Accessed April 16, 2018.
Note that if a fifth generation assay is used, differentiation of all three HIV analyte markers (antigen, HIV-1, and HIV-2 antibodies) occurs in the first step.

HIV Testing. Atlanta, GA: Centers for Disease Control and Prevention: US Department of Health and Human Services. Available at: http://www.cdc.gov/hiv/testing/index.html. Accessed April 16, 2018.