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Theoretical and Practical Aspects of Routine H&E Staining (Online CE Course)

(based on 23 customer ratings)

Author: Anita Buchiane, HT(ASCP) QIHC
Reviewer: Carla Shoffeitt, MSM, HT(ASCP)

This course will explore the history and application of hematoxylin and eosin (H&E) stains used in the clinical histology laboratory. Basic H&E staining mechanisms and the classification of biological stains will be discussed. The material presented details the chemistry, diagnostic application, and problem solving strategies for H&E stains.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-011-18, approved through 5/31/2020
Course number 20-658952, approved through 9/1/2018

Objectives

  • Differentiate between natural and synthetic dyes.
  • Analyze progressive staining versus regressive staining.
  • Distinguish between the oxidants and mordants in common hematoxylin and eosin (H&E) stains.
  • Identify cell constituents demonstrated with the H&E stain.
  • Describe the purpose of and reagents used for deparaffinization, hydration, and dehydration.
  • Identify potential problems encountered with routine staining and solutions to resolve them.

Customer Ratings

(based on 23 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Introduction
  • Categories of Biological Stains
      • Three Broad Categories of Biological Stains
      • Which one of the following categories of stains is used to differentiate between a cell's nucleus and cytoplasm?
      • Which category of stain would you employ if asked to demonstrate muscle in a tissue sample?
  • Classification of Biological Stains
      • Classification of Biological Stains
      • An acidic dye has a pH less than 7 and has an affinity for __________ tissue components.
      • Origin of Dyes: Natural
      • Origin of Dyes: Synthetic
      • The first natural dyes were made from aniline and are therefore sometimes referred to as aniline dyes.
      • Examples of Synthetic Dyes
      • Chemistry of Dyes
      • Mechanisms of Action
      • What is the staining mechanism that overstains tissue and then employs a differentiation step?
      • Progressive or Regressive Hematoxylin Staining
      • Which type of staining employs the process of differentiation to delineate desired tissue elements?
  • Routine Staining in the Histology Laboratory
      • Hematoxylin Oxidation
      • Mordants
      • Commonly used Hematoxylins: Their Oxidizers and Mordants
      • Differentiating
      • The Bluing Step
      • Eosin as a Counterstain
      • Types of Eosin
      • Eosin Differentiation
      • Match the following solutions with its' appropriate use.
      • One advantage of using alcohol-based eosins over aqueous eosins is that they will stain slower and produce a wider range of shades.
      • Other than the cytoplasm of cells, what other tissue constituents are stained with eosin? (Choose all that apply.)
  • Applications of the H&E Stain
      • Healthy Versus Diseased Tissue with H&E Stain
      • Uterus
      • Liver
      • Breast
      • Skin
      • Appendix
      • Fallopian Tube
  • Additional Steps Employed in Routine H&E Staining
  • Some Common Problems Encountered in H&E Staining
  • References
      • References

Additional Information

Level of instruction: Intermediate
Intended audience: Clinical laboratory histotechnologists and technicians,and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students,  pathology residents, and practicing pathologists. 
Author information: Anita Buchiane, HT(ASCP) QHIC, is the Lead Histology Technician at Brattleboro Memorial Hospital in Brattleboro, Vermont. Prior to this position, she worked as a Neuropathology/Special Procedures Technician at Baystate Medical Center in Springfield, Massachusetts.  Ms. Buchiane is a graduate of Hartford Hospital School of Allied Health in Connecticut, where she earned her Certificate in Histotechnology, and Greenfield Community College, Greenfield, Massachusetts, where she earned an associates degree in Liberal Arts with a math and science concentration. She is a member of the National Society for Histotechnology and the VT/NH Society for Histotechnology.  She was the recipient of the 2007 Lee G. Luna Foreign Travel Scholarship awarded by the National Society for Histotechnology and the 2010 Region 1 Scholarship awarded by the VT/NH  Society for Histotechnology.
Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP) is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.
Course Description: This course will explore the history and application of hematoxylin and eosin (H&E) stains used in the clinical histology laboratory. Basic H&E staining mechanisms and the classification of biological stains will be discussed. The material presented details the chemistry, diagnostic application, and problem solving strategies for H&E stains. 

breast 40x_edit
Breast H&E S11-792a with arrows
DD1_edit
Fallopian Tube H&E with arrows
kw3_edit
liver bx 40x H&E with arrows
not blued_edit
How to Subscribe
Histology CE Package$65 Add to cart
Individual course$20 Add to cart


breast 40x_edit


Breast H&E S11-792a with arrows


DD1_edit


Fallopian Tube H&E with arrows


kw3_edit


liver bx 40x H&E with arrows


not blued_edit