Respiratory Case Study: Possible Pertussis Infection (Online CE Course)

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Author: Leah Ames, MS, MLS(ASCP)^CM
Reviewer: Adelisa Franchitti, MS, MLS, Laurie Bjerklie, MA, MLS(ASCP)^CM

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This course describes the clinical symptoms of Bordetella pertussis infection. Proper specimen collection and transport for diagnosis of B. pertussis along with laboratory testing procedures that aid in its diagnosis are presented. Advantages and disadvantages for each testing procedure including culture, nucleic acid amplification testing (NAAT), direct fluorescent antibody staining, and serology are discussed. Treatment options for pertussis infection including available vaccines are presented in this course.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)

Approved through 9/30/2024

Florida Board of Clinical Laboratory Personnel Credit Hours - General (Molecular Pathology): 1 hour(s)

Approved through 9/30/2024

Objectives

Identify the clinical symptoms of Bordetella pertussis infection.
Describe proper specimen collection and transport for diagnosis of B. pertussis.
Identify laboratory testing procedures that aid in the diagnosis of B. pertussis.
Discuss advantages and disadvantages for each testing procedure including culture, nucleic acid amplification testing (NAAT), direct fluorescent antibody staining, and serology.
Explain the principles of NAAT testing for B. pertussis.
Discuss treatment options for pertussis infection.
Identify available vaccines that can help to prevent infection with B. pertussis.

Customer Ratings

(based on 196 customer ratings)

Course Outline

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Case Presentation

Case Study
Pathogenesis and Virulence Factors
Epidemiology and Transmission
Clinical Course and Significance of the Disease
Table 1: Stages and Clinical Features of Pertussis
Complications
Case Study, continuedThe clinician calls the laboratory to obtain instructions for the collection of the specimen. Which of these specimens and collec...
Proper Specimen Collection and Transport

Laboratory Workup

Identification by Culture Methods
Case Study, continued: Laboratory Testing
Case study, continued Upon arrival in the laboratory, one of the swabs is used for culture. Which one of these selective media is an appropriate choic...
Case Study, continuedAfter four days of incubation at 37°C, small, shiny colonies are observed on the Regan-Lowe agar. A Gram stain of the cultur...
Nucleic Acid Amplification Testing (NAAT)
Genomic Insertion Sequences
Single-Copy Gene Targets
Positive Predictive Value
Addressing False-Positive NAAT Results
True or false: Insertion sequences can move around and insert into different locations on the genome, changing the overall genetic sequence of the bac...
Direct Fluorescent-Antibody Staining (DFA)
White Blood Cell (WBC) Count
Case Study, continued: IgA, IgM and IgG Antibody Testing
Table 2: Advantages and Disadvantages of Laboratory Tests for Identification of B. pertussis
Clinical Stages of Pertussis and Optimal Timing for Diagnostic Testing
Which of these tests may be performed late in the course of B. pertussis infection?
How does the prevalence of a particular pathogen in a given area correlate to positive predictive values?

Case study conclusion

Case Study Conclusion
Treatment and Dosing for Pertussis
Prevention
Which of the following antibiotics is recommended for infants less than one month old for treatment of Bordetella pertussis infection?

References

References

Additional Information

Level of instruction: Intermediate

Intended Audience: Medical laboratory scientists, medical laboratory technicians, pathology residents, MLS students, and other health care personnel who have an interest in this subject matter.

Author information: Leah Ames, MS, MLS(ASCP)^CM is a medical technologist in the Molecular Diagnostics department at Alverno Clinical Laboratories. She also serves as adjunct faculty for molecular diagnostics in the CLS program at Northern Illinois University and is an instructor of molecular diagnostics for Franciscan St. Margaret Health School of Medical Technology. Leah holds an M.S. degree in Clinical Laboratory Science from Michigan State University and a B.S. in Molecular and Integrative Physiology from the University of Illinois.

Reviewer information:

Adelisa Franchitti, MS, MLS, is currently employed by General Leonard Wood Army Community Hospital Laboratory. Previous to this employment, she was an Instructor in the Department of Biochemistry, Kansas State University. Ms. Franchitti holds a Masters in Microbiology and a Masters in Molecular Biology.

Laurie Bjerklie, M.A., MLS(ASCP)^CM, is currently a Content Developer for MediaLab and LabCE. She has been in higher education for over 14 years, has been a professor in both MLT and MLS programs, and served as a Program Director.

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