Course Outline
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- Case Presentation
- Case Study
- Pathogenesis and Virulence Factors
- Epidemiology and Transmission
- Clinical Course and Significance of the Disease
- Table 1: Stages and Clinical Features of Pertussis
- Complications
- Case Study, continuedThe clinician calls the laboratory to obtain instructions for the collection of the specimen. Which of these specimens and collec...
- Proper Specimen Collection and Transport
- Laboratory Workup
- Identification by Culture Methods
- Case Study, continued: Laboratory Testing
- Case study, continued Upon arrival in the laboratory, one of the swabs is used for culture. Which one of these selective media is an appropriate choic...
- Case Study, continuedAfter four days of incubation at 37°C, small, shiny colonies are observed on the Regan-Lowe agar. A Gram stain of the cultur...
- Nucleic Acid Amplification Testing (NAAT)
- Genomic Insertion Sequences
- Single-Copy Gene Targets
- Positive Predictive Value
- Addressing False-Positive NAAT Results
- True or false: Insertion sequences can move around and insert into different locations on the genome, changing the overall genetic sequence of the bac...
- Direct Fluorescent-Antibody Staining (DFA)
- White Blood Cell (WBC) Count
- Case Study, continued: IgA, IgM and IgG Antibody Testing
- Table 2: Advantages and Disadvantages of Laboratory Tests for Identification of B. pertussis
- Clinical Stages of Pertussis and Optimal Timing for Diagnostic Testing
- Which of these tests may be performed late in the course of B. pertussis infection?
- How does the prevalence of a particular pathogen in a given area correlate to positive predictive values?
- Case study conclusion
- Case Study Conclusion
- Treatment and Dosing for Pertussis
- Prevention
- Which of the following antibiotics is recommended for infants less than one month old for treatment of Bordetella pertussis infection?
- References
Additional Information
Level of instruction: Intermediate
Intended Audience: Medical laboratory scientists, medical laboratory technicians, pathology residents, MLS students, and other health care personnel who have an interest in this subject matter.
Author information: Leah Ames, MS, MLS(ASCP)CM is a medical technologist in the Molecular Diagnostics department at Alverno Clinical Laboratories. She also serves as adjunct faculty for molecular diagnostics in the CLS program at Northern Illinois University and is an instructor of molecular diagnostics for Franciscan St. Margaret Health School of Medical Technology. Leah holds an M.S. degree in Clinical Laboratory Science from Michigan State University and a B.S. in Molecular and Integrative Physiology from the University of Illinois.
Reviewer information:
Adelisa Franchitti, MS, MLS, is currently employed by General Leonard Wood Army Community Hospital Laboratory. Previous to this employment, she was an Instructor in the Department of Biochemistry, Kansas State University. Ms. Franchitti holds a Masters in Microbiology and a Masters in Molecular Biology.
Laurie Bjerklie, M.A., MLS(ASCP)
CM, is currently a Content Developer for MediaLab and LabCE. She has been
in higher education for over 14 years, has been a professor in both MLT and MLS
programs, and served as a Program Director.
