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- Importance of Fluorescence In Situ Hybridization (FISH) Testing in Cancer Care
- Theoretical Knowledge Required for Fluorescence In Situ Hybridization (FISH) Testing
- Equipment used in FISH technology
- FISH methodology
- Tissue Preparation
- Common Methodologies
- Slide Preparation
- Pre-treatment Strategies
- Standard Pre-treatment
- Standard Protease Digestion
- Standard Denaturation
- Standard Probe Preparation and Hybridization
- Standard Post-Hybridization and Completion
- New Histologic-Based Fluorescence In Situ Hybridization (FISH) Technique
- Differences in Pre-treatment
- Differences in Digestion
- Which of the following pre-treatment solutions are more harsh to the tissue?
- It is acceptable to use the digital reading on the water bath during the FISH assay.
- It is important to denature the tissue DNA and the probe DNA.
- What happens during the hybridization step of a FISH assay?
- Enumeration of FISH signals
Level of instruction: Beginning to intermediate
Intended audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
Author information: Kathryn R. Perkinson, BS, HTL(ASCP) has been the manager of the Molecular Pathology Department of Duke University Health System in Durham, North Carolina since 1998. She holds a Bachelor of Science degree in biochemistry from North Carolina State University. Kathryn has over 25 years of experience in molecular biology in both the research and clinical healthcare fields at Duke University. The Molecular Pathology Department, under her leadership, has tripled in size over the past 15 years.
Reviewer information: Jim Burchette, HT(ASCP) recently retired after 34 years at Duke University Medical Center, Durham, North Carolina. As an Immunopathology Analytical Specialist in Molecular Pathology, Jim's primary job functions included daily IHC quality control and managing the daily technical aspect of the Clinical Immunopathology operation. He has over 38 years of experience in surgical pathology histotechnology with 33 years of immunopathology specialty. Jim has an extensive experience validating new antibodies for research and diagnostic immunohistochemical use. To date, Jim has 80 publications (70 of which are peer-reviewed) and two book chapters, as well as many abstracts, posters, and technical acknowledgments in peer reviewed scientific articles. He has also served as a consultant for Triangle Biomedical Sciences (TBS), Dako North America, Leica Microsystems, and Polysciences.
Course description: This course will explore the theoretical concepts used in fluorescence in situ hybridization (FISH) testing. Commonly used FISH methodologies, necessary equipment, and the enumeration of FISH signals will also be discussed.