Course Outline
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- Importance of Fluorescence In Situ Hybridization (FISH) Testing in Cancer Care
- Theoretical Knowledge Required for Fluorescence In Situ Hybridization (FISH) Testing
- Equipment used in FISH technology
- FISH Methodology
- Tissue Preparation
- Common Methodologies
- Slide Preparation
- Pre-treatment Strategies
- Standard Pre-treatment
- Standard Protease Digestion
- Standard Denaturation
- Standard Probe Preparation and Hybridization
- Standard Post-Hybridization and Completion
- New Histologic-Based Fluorescence In Situ Hybridization (FISH) Technique
- Differences in Pre-treatment
- Differences in Digestion
- Which of the following pre-treatment solutions are more harsh to the tissue?
- True or False: Using the digital reading on the water bath during the FISH assay is acceptable.
- True or False: It is important to denature the tissue and probe DNA.
- What happens during the hybridization step of a FISH assay?
- Enumeration of FISH signals
- References
Additional Information
Level of instruction: Beginning to intermediate
Intended audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel interested in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
Author information: Kathryn R. Perkinson, BS, HTL(ASCP), has been the manager of the Molecular Pathology Department of Duke University Health System in Durham, North Carolina, since 1998. She holds a Bachelor of Science in biochemistry from North Carolina State University. Kathryn has over 25 years of experience in molecular biology in both the research and clinical healthcare fields at Duke University. The Molecular Pathology Department, under her leadership, has tripled in size over the past 15 years.
Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP), is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management and a Bachelor of Science in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.
Course description: This course will explore the theoretical concepts used in fluorescence in situ hybridization (FISH) testing. It will also discuss commonly used FISH methodologies, necessary equipment, and the enumeration of FISH signals.
